Влияние источников азота в питательной среде на рост и целлюлазную активность стрептомицетов

Abstract

Biomass accumulation and cellulase activity were determined in two promising soil isolates during liquid phase cultiva-tion depending on organic (peptone, casein hydrolysate) and mineral (NH4Cl, NaNO3, NH4NO3, (NH4)2SO4, KNO3) nitrogen sources. The only source of carbon in the medium was straw (1ob.%). Using two-factor analysis of variance, it was found that the production of cellulase complex enzymes is equally influenced by the nitrogen source (F= 225,92; p<0,0001), the producer strain (F=205,98; p<0,0001), the interaction of these factors (F=225,06; p< 0,0001). Inorganic nitrogen sources were the most preferred for cellulase production: NH4NO3 provided enzymatic activity in Streptomyces sp. Mb 4-2 is 912,82±60,40 units/10 min /g, and NH4Cl in Streptomyces sp. 1.3 NH4Cl is 531,37±27,25 units/10 min /g, which is 58–481 % and 34,5–409% more, respectively, than on other tested nitrogen sources. The accumulation of biomass during growth was more dependent on the nitrogen source (F= 65,47; p<0,0001) than on the strain of streptomycetes (F=18,65; p<0,0001). The interaction of these factors did not have a significant effect on the accumulation of biomass (F=1,11; p=0,38). Peptone and casein hyd-rolysate became the best nitrogen source for the growth of streptomycetes, providing an average of 19 % more biomass than inorganic nitrogen sources. It is shown that the optimization of the nutrient medium by the nitrogen source makes it possible to regulate the growth rates of streptomycetes-producing cultures and achieve maximum cellulase yield. The results obtained are of interest for the development of new environmentally friendly technological processes using streptomycetes in the production of cellulase complex enzymes and in the destruction of cellulose-containing agricultural waste.