Определение пула каротиноидов с использованием резонансной рамановской спектроскопии у беременных с преэклампсией

Abstract

Objective. To assess the performance of resonance Raman spectroscopy in the evaluation of the carotenoid pool (antioxidants) in plasma of pregnant women with preeclampsia (PE). Patients and methods. We examined 60 pregnant women aged between 21 and 41 years, including 30 women with moderate PE, 18 women with severe PE, and 12 women with normal pregnancy. We used Raman spectroscopy to analyze serum specimens. Results. We evaluated diagnostic accuracy of Raman spectroscopy (as a method of express diagnostics) and measured normalized integrated Raman scattering intensity for carotenoids in women with moderate preeclampsia (5.0 ± 0.2), severe preeclampsia (6.1 ± 0.3) and normal pregnancy (10.1 ± 0.6). We also separated Raman luminescent spectra of plasma specimens obtained from patients in different groups: the accuracy of distinguishing between normal pregnancy and PE was 96%, while the accuracy of distinguishing between moderate PE and severe PE was 72%. Conclusion. Raman spectroscopy is a relatively simple, but highly effective method for rapid estimation of carotenoid levels in plasma of pregnant women with various grades of PE. Our findings suggest that Raman spectroscopy is a good method of preclinical diagnosis and prognosis of PE, particularly in women at high risk. Key words: carotenoids, discriminant analysis, preeclampsia, Raman spectroscopy, rapid diagnostics