МОРФОЛОГИЯ ПОПУЛЯЦИЙ САЛЬМОНЕЛЛ В ВОДНОЙ СРЕДЕ И ИХ СПОСОБНОСТЬ К ФОРМИРОВАНИЮ БИОПЛЕНОК

Abstract

The aim of the work is to study the survival strategy of salmonella populations in water medium with the formation of biofilms and L-transformation processes. Materials and methods. Salmonella typhimurium №1957 was chosen as a test culture. For the quantitative account of the growth of Salmonella and their morphology, an accelerated method of serial dilution was used with a seeding of 0,05 ml on membrane filters No. 4-5 production «Vladipor» placed on the dense nutrient medium of the BSA. Biochemical properties were determined by accelerated method using MTS-Salm system. For SEM, the preparations were fixed with an aqueous solution of glutaraldehyde, dehydrated with propylene oxide. The preparations were sprayed with platinum or gold ions on the «Hitachi-E-102» installation. The work was carried out on a scanning electron microscope Hitachi TM3030. Research results. The study of Salmonella preparations from water samples in vitro by method SEM showed that after 7 days of Salmonella existence in water at temperature of 26 ° C, the populations were united in microcolonies, covered from all sides by dense biofilm. After 2 months, the main part of the salmonella population was represented by the formed multilayer biofilm. After 6 months (observation period) at 26°C, the population of Salmonella cells was at the stage of L-transformation.