ВИЗНАЧЕННЯ ТОКСИКОЛОГІЧНИХ ВЛАСТИВОСТЕЙ, УЛЬЦЕРОГЕННОЇ ТА МІСЦЕВОПОДРАЗНЮВАЛЬНОЇ ДІЇ СУХИХ ЕКСТРАКТІВ ЛИСТЯ ТА КОРЕНЕВИЩ IRIS HUNGARICA

Abstract

Key words: acute toxicity, ulcerogenic and local irritating effect, acetylsalicylic acid, extract hungarian iris. In addition to high pharmacological activity, one of the most important requirements for drugs is their safety. The study objective was to the acute toxicity LD50 of dry extract of leaves and rhizomes of Hungarian iris when administered intragastrically and intraperitoneally to mice and intraperitoneally to rats, as well as possible ulcerogenic and local irritating effects. The study of the acute toxicity of dry extract of leaves and rhizomes of Hungarian iris was carried out according to the method of B. M. Shtabsky; a study of a possible ulcerogenic effect on the mucous membrane of the stomach and duodenum was carried out on Wistar rats according to the method of J. Marazzi-Uberti. A study of the effect of dry extract of leaves and rhizomes of Hungarian iris and acetylsalicylic acid on the stomach and intestines of animals under conditions of ethanol-induced gastric ulcers were carried out on rats for 14 days. The study of the local irritating effect of the dry extract of leaves and rhizomes of hungarian iris was carried out on rabbits of the Chinchilla breed. When studying the acute toxicity of the dry extract of leaves and rhizomes of Hungarian iris when administered intragastrically to mice (at doses of 500 mg / kg, 5000 mg / kg, 10000 mg / kg and 15000 mg / kg) and intraperitoneally to rats (at doses of 10 mg / kg, 100 mg / kg, 1000 mg / kg, 3000 mg / kg and 5000 mg / kg) it was found that the death of experimental animals did not occur. Dry extract of leaves and rhizomes of Hungarian iris belongs to the V class of toxicity of substances - practically non-toxic substances, according to the classification of toxicity of substances by K. K. Sidorov. Dry extract of leaves and rhizomes of Hungarian iris does not show ulcerogenic action at doses of 50 mg / kg and 150 mg / kg. An insignificant ulcerogenic effect of dry extract of leaves and rhizomes of Hungarian iris on the gastric mucosa of rats was revealed only at a dose of 200 mg / kg. On the model of ethanol stomach ulcers, dry extract of leaves and rhizomes of Hungarian iris did not potentiate the ulcerogenic effect of ethyl alcohol. Dry extract of leaves and rhizomes of Hungarian iris does not show a local irritating effect on the mucous conjunctiva of the eye of rabbits.