용매 종류와 효소 처리에 따른 쓴 메밀 추출물의 항산화 활성 및 α-Glucosidase 저해 활성의 변화

Abstract

Extract yield of tartary buckwheat treated with water, 70% ethanol or methanol were about 13.6%, 7.0% and 6.6%, respectively. Extract yield was greatly increased by the treatment of α-amylase indicating 95.1% yield. RC?? value of DPPH radical scavenging activity with methanol and 70% ethanol extracts were 34.0 ㎍/mL, 40.5 ㎍/mL, respectively. The DPPH radical scavenging activity increased when it was treated with β-glucosidase and cellulase, showing RC?? value of 24.7 ㎍/mL and 25.0 ㎍/mL, respectively. In ABTS radical scavenging activity, methanol extract (100 ㎍/mL) showed 30% inhibition. In DPPH or ABTS radical scavenging activities, the treatment of β-glucanase and α-amylase shows the highest and the lowest activities, respectively. In α-glucosidase inhibitory effect, 70% ethanol extract showed RC?? value of 59.9 ㎍/mL, but water extract was not inhibitory effective. The α-glucosidase inhibitory effect was the highest in multi enzyme treatment. Content of rutin and quercetin in methanol extract showed higher value with 4400.3 mg% and 71.9 ㎎%, respectively. The 70% ethanol extract of buckwheat contained rutin of 3459.8 ㎎% and quercetin of 56.9 mg%. In the treatment of β-glucanase, the rutin content of ethanol extract increased with 5057.4 ㎎% and multi-enzyme treatment resulted in the modification of rutin glycoside.