Стандартизация сухого экстракта сока мякоти тыквы, обладающего гиполипидемическим действием по содержанию β-каротина

Abstract

The objective is to standardize the content of β-carotene – one of the principal active substances with sufficient hypolipidemic activity in the resultant original product. Search for natural biologically active substances having a hypolipidemic effect is one of the topical questions in modern pharmacy. Most of the research is carried out by means of isolating individual substances from the plant object. We propose to study a native phytocomplex obtained from pumpkin pulp – a dry extract of pumpkin pulp juice. The following materials and methods were used: laboratory-obtained dry extract of pumpkin pulp juice and methods used to determine the qualitative and quantitative content of β-carotene in an individual substance. It was possible to adapt the methods applied to the analysis of an individual substance in relation to the resulting phytocomplex containing both lipophilic and hydrophilic fractions. The rapid method of quantitative determination of β-carotene described in the scientific literature was corrected. An effective alternative method for spectrophotometric determination of β-carotene in the phytocomplex has been developed. Phytocomplexes standardized for the main active substances can be used to obtain effective drugs and biologically active additives with minimal side effects. In addition, the expansion of the search for alternative natural objects containing complexes haaving a hypolipidemic effect is currently relevant. Based on the literature data, in the future it is planned to conduct a pharmacological study to establish the anti-inflammatory effect of the resulting complex on the state of the vascular wall.