ДИНАМИКА ИЗМЕНЕНИЙ ПОКАЗАТЕЛЕЙ ОКИСЛИТЕЛЬНОГО ГОМЕОСТАЗА В ПРОЦЕССЕ РЕПЕРФУЗИИ ПЕЧЕНИ КРЫС ПОСЛЕ ВАСКУЛЯРНОЙ ЭКСКЛЮЗИИ

Abstract

Changes in the state of the antioxidant protection system and the intensification of free radical processes are the leading pathobiochemical mechanism for the development of reperfusion damage to cells. The aim of this paper is to determine the features of changes in markers of oxidative stress at different periods of development of the acute reperfusion after total vascular exclusion of rat liver. The study was carried out on 100 white nonlinear sexually mature male rats weighing of 200-250 grams. The animals of the experimental groups were simulated with a 20-minute vascular isolation of the liver. Blood in a volume of 2-3 ml was taken from the inferior vena cava for 5, 15, 30, 60, 120, 180 minutes, 8 hours and a day after the restoration of blood flow. The results of the study showed that in the early stages of the reperfusion period (5-60 minutes), changes are observed due to cytolysis of hepatocytes during the period of organ ischemia and leaching of cell contents into the blood. The level of radical sorption of ABTS by blood plasma briefly increased by 20% by the 5th minute of the reperfusion period; at the same period, an increase in glutathione peroxidase activity by 2.5 times was noted. The total antioxidant activity, determined by the iron-reducing method, was increased by 2 times within the period of 15-30 minutes after the restoration of blood flow. The main manifestations of reperfusion damage to the liver developed in 60 minutes after the restoration of blood flow and were characterized by a sharp decrease in total AOA by 1.5-2 times and the concentration of reduced glutathione by 20%. The system of antioxidant protection of blood plasma was the first to react to the spread of the pathological process at the systemic level and its changes persisted one day after liver ischemia. A 20-minute vascular exclusion of the liver was accompa- nied by changes in the antioxidant balance in the erythrocyte suspension, but these changes returned to normal a day after the restoration of blood flow. The data obtained make it possible to distinguish between the contribution of ischemic and reperfusion injuries to the development of oxidative disorders, which is important from the standpoint of assessing the possibilities of anti- oxidant therapy.