Мониторинг быстрых вариаций содержания эндогенного СО в выдыхаемом воздухе методами диодной лазерной спектроскопии

Abstract

A.I.Dyachenko 1, E.V.Stepanov 1, Yu.A.Shulagin 2 1 Prokhorov General Physics Institute of the Russian Academy of Science 2 Institute of Biomedical Problems of the Russian Academy of Science Metods of tunable diode laser spectroscopy were used to monitor fast physiological variations of endogenous carbon monoxide (CO) content in expired air. For these purposes, a CO analyzer was created on the basis of a tunable diode laser emitting in the region of 2.35 μm at room temperature. To determine the concentration of CO in exhaled air, the value of the resonance absorption of this molecule in the R (6) line of the 0–2 band located near 4285 cm – 1 was measured. The use of this line, which is well isolated from nearby H2O absorption lines, allows one to ensure high selectivity of CO analysis in conditions of high humidity of the analyzed air. The capabilities of monitoring rapid physiological variations in the CO content in the exhaled air of humans and animals using the developed system were demonstrated in studies using different physiological stress tests, such as pure oxygen breathing (both in humans and rats), physical activity, and hyperventilation. The dynamics of the allocation of CO due to the use of certain pharmaceuticals were also observed. It was shown that laser analysis of CO in the spectral range of 2.35 μm makes it possible to detect fast dynamic processes of the evolution of this gaseous molecule, which is one of the signal metabolite molecules, during various physiological and medical studies, to study the patterns and relationships of the processes responsible for production, transport, buffering and release of CO in the body.