Abstract
Aim. The classic detachment techniques lead to changes in cells properties. We offer a simple method of cultivating the population of cells that avoided an influence on the surface structures. Methods. Mesenchymal stem cells (MSC) from human umbilical cord matrix were obtained and cultivated in standard conditions. While substituting the culture media by a fresh portion, the conditioned culture medium, where the cells were maintained for three days, was transferred to other culture flacks with addition of serum and growth factors. Results. In the flacks, one day after medium transfer, we observed attached cells with typical MSC morphology. The cultures originated from these cells had the same rate of surface markers expression and clonogenic potential as those replated by standard methods. Conclusions. MSC culture, derived by preserving the cells with reduced attachment ability, actually has the properties of «parent» passage. Using this method with accepted techniques of cells reseeding would allow maintaining the cells that avoided an impact on the cell surface proteins.
Highlights
IntroductionMesenchymal stem cells (MSC) are considered to be one of the most promising instruments of cell therapy
Mesenchymal stem cells (MSC) are considered to be one of the most promising instruments of cell therapy. Their cultivation is related to some difficulties, which have not been overcome to a full degree
In the tissues of organism MSC are located in the complex three-dimensional system, where their surface apparatus is adjusted to receive and process the signals from outside
Summary
Mesenchymal stem cells (MSC) are considered to be one of the most promising instruments of cell therapy. Their cultivation is related to some difficulties, which have not been overcome to a full degree. The methods of isolating MSC from tissues, their cultivation, passaging, and facilitating the retention of multipotency are actively elaborated and improved [1,2,3]. In the tissues of organism MSC are located in the complex three-dimensional system, where their surface apparatus is adjusted to receive and process the signals from outside. The extracellular matrix and surrounding cells create unique physical and chemical conditions, determining MSC behavior [4, 5]. During the cultivation in the monolayer the cells get accustomed to specific conditions and released from centralized regulation by the neuroimmune and humoral factors [6]
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