Abstract
Mesenchymal stem cells (MSCs) are often used in the experimental studies of the enhancement of damaged nerve regeneration. The aim of this work was to study the effect of subperineural transplantation of MSCs on the regenerating fibers of a damaged sciatic nerve in rats using immunohistochemical detection of peripherin. The suspension bone marrow-derived mesenchymal stem cells(5x104 cells in 5 μl of medium) from Wistar-Kyoto rat was transplanted into the rat sciatic nerve damaged by ligature application (40 s). 5 μl of culture medium was injected subperineurally into the animals in the control group following the ligature application. Two months after the operation, peripherin-immunopositive nerve fibers were counted and measured on transverse sections of the distal segment of the recipient's nerve. Morphometric analysis of regenerating fibers was performed using the ImageJ software (NIH, USA). It showed that the average thickness of nerve fibers in animals of the experimental group was increased. A study of the thickness of the nerve fibers of the distal segment of the damaged nerve showed that in animals treated with MSCs, the percentage of larger diameter fibers was higher in comparison with the control group.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
