Наноскальпель на основе магнитных дисков и аптамеров эффективно и адресно разрушает опухолевые ткани

Abstract

The aim of the research. To investigate the antitumor effi cacy of three-layer magnetic nanodiscs (Au / Ni / Au) with a quasi-dipole structure, functionalized with biorecognizing molecules of a tumor. Material and methods. Th ree-layer magnetic nanodiscs (Au / Ni / Au) 500 nm in size (were obtained by micro- and nanoelectronic technologies. Aptamers to ascites cells of Ehrlich carcinoma were used to functionalize magnetic nanodiscs. Th iol groups were used to bind disks to aptamers. As a model of tumor cells Ehrlich’s ascites carcinoma cells were used, and the cells were magnetically infl uenced by an alternating magnetic fi eld (50 Hz, 100 Oe). Results. Magnetic nanodiscs have magnetic anisotropy, which proves their high sensitivity to magnetic stimuli. Magnetic nanodiscs start the processes of cell death in the culture of ascites cells for two hours. Presumably, magnetic nanodiscs use aptamers to bind to the cell membrane protein fi lamin A, a structural component of the cytoskeleton that plays an important role in cell signaling and, when exposed to a variable magnetic, cause destruction of the cell membrane and cell death. Conclusion. Microsurgery of malignant tumors using a nanoscalpel based on functionalized ligands that recognize tumor sites can be used to remove single tumor cells during surgery