Abstract
본 연구는 칸디다 알비칸스(C. albicans)에 대한 프로폴리스의 억제효과를 확인하고자 수행되었다. 프로폴리스는 양봉 꿀벌에서 채취하여 C. albicans는 <TEX>$37^{\circ}C$</TEX>에서 액체 배지에서 2시간 배양 하였다. 항균 활성검삼를 위해서 생리식염수 (PBS), 3% 차아염소산나트륨 (NaOCl에), 0.1% 클로로헥시딘 (CHX), 프로 폴리스 추출물 (<TEX>$5{\mu}L/m{\ell}$</TEX>, <TEX>$10{\mu}L/m{\ell}$</TEX>)에서 평가하였다. C. albicans는 3%의 NaOCl, 0.1% CHX, 프로 폴리스(<TEX>$5{\mu}L/m{\ell}$</TEX>, <TEX>$10{\mu}{\ell}/m{\ell}$</TEX>)에서 15, 14.5, 16, 17mm의 억제구간을 확인하였다. 항균활성을 확인하기 위하여 집락형성을 분석한 결과, 3%의 NaOCl, 0.1 % CHX, <TEX>$5{\mu}L/m{\ell}$</TEX>와 <TEX>$10{\mu}L/m{\ell}$</TEX>의 프로 폴리스가 7, 7, 5, 7 로그지수가 감소를 보였다. C. albicans서는 <TEX>$10{\mu}L/m{\ell}$</TEX>의 프로폴리스에서 유의하게 억제되는 결과를 보여주었다. 이러한 결과를 통하여 프로폴리스는 구강 점막 질환에서 새로운 향균 약제가 될수 있는 것으로 생각된다. Propolis is an extremely safe natural antimicrobial substance that has been reported to have powerful antibacterial efficacy. The aim of this study was to evaluate the inhibitory effects of propolis against Candida albicans (C. albicans). Propolis was collected from the honey bee Apis mellifera. The strain of C. albicans was cultivated overnight in liquid media incubated at <TEX>$37^{\circ}C$</TEX>. The antimicrobial activity was investigated using phosphate buffered saline (PBS), 3% sodium hypochlorite (NaOCl), 0.1% chorhexidine (CHX), and propolis extracts (<TEX>$5{\mu}l/ml$</TEX>, <TEX>$10{\mu}l/ml$</TEX>). C. albicans were sensitive to 3% NaOCl, 0.1% CHX, and propolis (<TEX>$5{\mu}l/ml$</TEX>, <TEX>$10{\mu}l/ml$</TEX>) with zones of inhibition of 15, 14.5, 16, and 17 mm, respectively. The CFU of PBS, 3% NaOCl, 0.1% CHX, <TEX>$5{\mu}l/ml$</TEX> and <TEX>$10{\mu}l/ml$</TEX> of propolis led a 1, 7, 7, 5 and 7-log reduction. Among the groups tested, C. albicans was most sensitive to <TEX>$10{\mu}l/ml$</TEX> of propolis, which showed the largest inhibition zones. Therefore, propolis can be a new antimicrobial therapy for oral mucosa disease in traditional medicine.
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