Влияние природы активных компонентов и модификаторов на электроаналитические свойства планарных цефалексин-селективных сенсоров

Abstract

The 1st generation cefalexine-cephalosporine antibiotic is used in the treatment of various infectious diseases. Spectrophotometry, kinetic spectrophotometry, spectrofluorimetry are proposed to determination of cefalexine in medicine and biological environment. Planar screenprinted sensors allow analyzing the micro-volumes of samples, which is important for the analysis of biological objects without preliminary samplepreparation. Depending on the active material and modifiers, you can create planar sensors for the determination of different organic compounds. In this work we have studied the influence of the nature of electroactive compounds and modifiers on the electroanalytic properties of planar cefalexine-selective sensors. Associates of tetradecylammonium and dimethyldistearylammonium with complex compounds silver (1) – cefalexine (Ceas = 1–3%), polyaniline modifiers and cupric oxide nanoparticles have been used as active components, the ratio EAS: modifier is 1:1. The main electroanalytic and operational characteristics of cefalexine-selective sensors in aqueous solutions and on the background of oral fluid are determined. Advantage of tetradecylammonium in active components of cefalexine-selective sensors is shown. For cefalexine- sensors, the optimal is: linearity interval 1·10-2 – 1·10-4, response time 20–25 seconds, for unmodified: 10–15 sec, for modified in 1·10-2 M solutions of cefalexine, service life – 1 month. Modifiers approximate angular coefficients of electrode functions to theoretical values for single-charge ions, reduce response time and drift of potential, reduce the detection limit of cefalexine. Sensors are used for the determination of cephalexine in model aqueous solutions and oral fluid with added antibiotic additives, in expired cephalexine preparations.