Кластерний аналіз впливу флуренізиду на показники стану прооксидантно-антиоксидантної системи зародків в’юна

Abstract

Experiments were carried out on embryos of Misgurnus fossilis L. After washing zygotes were incubated in physiological Golferre solution, containing a solution of flurenizide in concentrations of 0.01; 0.05; 0.15; 1; 5; 15 mM. The embryos were harvested for 60, 150, 210, and 330 minutes after fertilization of the 1 st (2 blastomeres), 4 th (16 blastomeres), 6 th (64 blastomeres), 8 th (256 blastomeres) and 10 th (1024 blastomeres) crushing egg zygotes. The intensity of lipids peroxide oxidation processes was evaluated by measuring of the content of lipid hydroperoxides and TBA-reactive substances. The state of antioxidant system was studied with the activity of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase. Cluster analysis was conducted to identify stages of development that have the same changes of the content of lipid hydroperoxides, TBА-reactive substances, activity of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase. Applying a cluster analysis it was found that before the stage of 64 blastomeres, all experimental groups were divided into 4 groups of similarity according to the studied indicators. Flurenizide in concentration of 0.05 mM in stage of 2 blastomers was found to have no significant effect on values whose were at the control level. Fluinizide in concentrations of 5 and 15 mM results in a significant decrease (compared with control) in the content of TBА-reactive substances, increasing of content of lipid hydroperoxides and decreasing of activity of catalase, glutathione peroxidase and glutathione-S-transferase. At the stage of development of 256 and 1024 blastomeres, all groups are divided into 5 groups of similarity, indicating change in the effect of flurenizyd on these stages of loach embryos development. In particular, at the development stage of 1024 blastomeres, flurenizide in a concentration of 1 mM leads to more pronounced increase in intensity of free radical processes, as well as the decrease in activity of superoxide dimitase, catalase, glutathione peroxidase and glutathione-S-transferase. It is known that this concentration is a daily therapeutic dose for an adult. It is likely that it is harmful to the germinal cells of liver, since it stimulates lipid peroxidation and suppresses antioxidant system ensymes. It has been established that with the action of flurenizide in concentrations of 5 and 15 mM, the content of hydroperoxides increases, the activity of superoxide dimitase, glutathione peroxidase and glutathione-S-transferase decreases. However, this effect is less pronounced compared with action of flurenizide in concentration of 1 mM.