Степень активации гемопоэза с помощью суммарной РНК зависит от способа ее парентерального введения

Abstract

The effects of hematopoiesis-stimulating total RNA were studied with subcutaneous (s/c), intramuscular (i/m), intraperitoneal (i/p), intravenous (i/v) and intranasal (i/n) routes of administration. Total RNA was isolated from the lymphoid cells of the spleens of healthy rats 17 hours after blood loss. Benzene anemia was used as a model of inhibited erythropoiesis. 28 days after the first benzene injection, RNA was introduced into rats. After 7 days in i/p, i/v, i/n groups the number of reticulocytes and platelets increased 1.5-2 times, in i/v and i/n groups the number of leukocytes increased 1.4-1.6 times. After 14 days in i/p, i/v, i/n groups the number of reticulocytes increased 2-2.6 times, the number of leukocytes - 1.6 times, platelets - 1.4-1.6 times. After 21 days in these groups, the number of reticulocytes was 3-4 times higher than the control rats, leukocytes - 1.4 times, platelets - 1.5-1.7 times. After 21 days the effect of the intramuscular administration of RNA appeared: the number of reticulocytes increased 2 times, the number of platelets - 1.2 times. On 21 days in rats were examined myelograms. With all groups, except «s/c», the number of basophilic, polychromatophilic and orthochromatic erythroblasts, as well as the number of megakaryocytes, significantly increased in the bone marrow. The number of myeloblasts, promyelocytes, myelocytes and lymphocytes increased in the bone marrow of rats only in i/p, i/v, i/n groups. Thus, the best methods for introducing total RNA were intraperitoneal, intravenous and intranasal routes of administration. With the intramuscular introduction, the stimulating hematopoiesis effect appeared only by 21 days. Sbcutaneous rout of administration administration of total RNA was ineffective.