Abstract
Толочь или резать? О методах пробоподготовки кератиновых материалов для анализа стабильных изотопов водорода
Highlights
Analysis of stable hydrogen isotopes (δ2H) in hair and other keratin-containing tissues is applied widely
We have examined the water sorption / desorption dynamics in the human hair and hair of domestic mammals that were grinded in different ways in order to determine whether this affected the amount of sorbed water
The samples belonging to the groups nos. 2 and 4 were taken for the further study, they were characterized by the largest and the lowest variability of values, respectively. Half of these samples were equilibrated with normal water (A2L, B2L, A4L, B4L), half, with heavy water (A2H, B2H, A4H, B4H) (Table 3, Fig. 3)
Summary
Analysis of stable hydrogen isotopes (δ2H) in hair and other keratin-containing tissues is applied widely. Despite the high accuracy of analysis and ease of handling of modern mass spectrometers, the analysis of stable hydrogen isotopes (δ2H) in keratin materials is a rather complicated task itself. The influence of the atmospheric water on the analyzed material is the main technical difficulty when applying this method. Hair is a very hygroscopic material; it quickly sorbs atmospheric moisture up to 10–20% of its dry weight. The hydrogen of keratin uncontrollably exchanges with the hydrogen of water molecules in the environment (Bowen et al, 2005; Meier-Augenstein and Kemp, 2012; Wassenaar and Hobson, 2000)
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