Влияние геотермальной воды в составе твердых сред культивирования на морфологические свойства дрожжей рода Saccharomyces

Abstract

The influence of geothermal waters located on the territory of the city of Makhachkala (Republic of Dagestan), in the composition of solid cultivation media, on the cultural and morphological features of genus Saccharomyces yeast was studied. Changes in cell properties and the formation of specific parameters of giant yeast colonies S. cerevisiae Y-503, S. cerevisiae DAW-3a, S. oviformis M-12x have been established. For YPD and PD media, as a result of a series of experiments, optimal options were selected containing geothermal and distilled water in a ratio of 1:1 (well No. 36) and 2:1 (well No. 26). It was shown that the YPD medium was optimal for yeast growth, while in the population of the tetraploid Y-503 strain, the average cell size was slightly larger than cell strain M-12x and significantly exceeded the DAW-3a values. Installed that the use of diluted water from well No. 36 in the cultivation medium is most effective and leads to accelerated growth of yeast colonies. In the variant geothermal / distilled water, this indicator exceeded the control by 1.3 (Y-503, M-12x) times; in the variant with geothermal water without dilution - by 1.6 (Y-503) and 1.4 (M-12x) times. The cells of the S. cerevisiae Y-503 strain, regardless of the medium used, are characterized by the presence of various forms, including elongated ones; DAW-3a - exceptionally rounded; S. oviformis M-12x - rounded and oval shapes. A comparative analysis of the parameters of the colonies showed that all variants are characterized by a rounded shape, a radially striated surface; flat, with a convex center profile; light beige color and pasty, spreadable texture. As a result of our research, a modified nutrient medium PD has been developed - an agar for growing yeast, containing geothermal water from well No. 36 of the Makhachkala field as an additional source of mineral and organic compounds. The proposed composition of the medium is of interest for its further use in microbiological studies.