Функціональна активність гемопоетичних клітин-попередників кордової крові у довготривалій культурі на гідрогелевій підложці in vitro

Abstract

Development of new and improvement of existing models of long-term cultivation of hematopoietic stem cells (HSC) and cord blood progenitor cells is one of the most important directions in modern cell biology, related to obtaining a sufficient amount of hematopoietic tissue for experimental and clinical use. The aim of the work was to investigate the functional activity of hematopoietic progenitor cells from cord blood during long-term cultivation on a soft polyacrylamide gel substrate in the presence of a cytokine complex and to determine the advantages of this method of maintaining hematopoiesis for further use in transplantation. To realize this purpose, the following methods were used: the method of long-term cultivation in vitro on a hydrogel substrate, the method of colony formation in semi-liquid agar, cytological research methods, light and inverted microscopy, statistical research methods. Due to the use of the hydrogel substrate together with the cytokine complex, a high proliferative activity of hematopoietic cells is observed, which is reflected in their expansion during long-term cultivation (5 weeks), as well as of high colony-forming activity (521.5 ± 7.5.105 per explanted cells). Thus, the stiffness of the substrate must be taken into account for the expansion of stem cells and their immediate descendants. The presence of a soft substrate made of polyacrylamide gel along with a complex of cytokines ensures the expansion of hematopoietic cells due to the long- term support of hematopoiesis.