Изучение разработанных внутрироговичных гелевых окрашенных имплантатов для кератопигментации на основе различных материалов. Экспериментальное исследование

Abstract

Purpose. Evaluation of the impact of the developed intracorneal gel stained implants for keratopigmentation based on various materials on the donor human cornea during organotypic cultivation. Material and methods. Three experimental samples of intracorneal gel stained implants were investigated: sample 1 based on sodium hyaluronate with organic pigment, sample 2 based on collagen hydrolyzate with inorganic pigment, and sample 3 based on hydroxypropyl methylcellulose (HPMC) with organic pigment. To determine the toxicity of the studied implants the apoptosis of keratocytes was determined in cryostat sections of the cornea. The method of immunohistochemistry was used to study apoptosis. Scanning electron microscopy was used to visualize the corneal structures in the presence of implants. Results. During the investigation we showed that a gel implant based on a collagen hydrolyzate and a pigment with an inorganic toner (28% in the composition) causes a weak expression of the initiator proteins of apoptosis Caspasa 8 and Cytochrome C, and there is no expression of the BAX and effector proteins Caspasa 3/7. It was revealed that samples No. 1 and No. 3 undergo partial dissolution and washout from the intrastromal tunnel, sample No. 2 based on collagen hydrolyzate has a dense structure and remains in the corneal tunnel throughout the entire cultivation period, for at least 7 days, which is showed using an electron-scanning microscopy. The proposed collagen hydrolyzate gel implant can be considered compact and non-toxic. Conclusion. As a result of organotypic cultivation for 7 days showed the best results intracorneal colored implant № 2 based hydrolyzate of collagen and inorganic toner. Intracorneal colored implant based on collagen hydrolyzate has a more compact and dense structure than the accompanying experimental samples. Key words: keratopigmentation, aniridia, intracorneal colored implant.