Abstract
Proteins are essential biomolecules that govern cellular activities and play a crucial role in understanding the characteristics of living organisms. A proper understanding of proteins is essential for comprehending the life sciences at the molecular level. In this study, our objective is to provide an experimental educational program for science-gifted middle school students to grasp the concepts of protein-based molecular biology. The experiments we have designed aim to analyze proteins from various organisms, helping students gain insights into their characteristics. To achieve this, we extract proteins from Escherichia coli, yeast, and human plasma/blood cells, and then separate total proteins by size using protein electrophoresis after adjusting their concentrations. Subsequently, we qualitatively determine the quantity and size of proteins through protein staining/de-staining processes. This is followed by discussions and comprehension of the major proteins in relation to biological characteristics. The entire experiment takes approximately 6 hours, allowing participants to learn diverse and universally applicable molecular experimental techniques related to protein analysis. To assess the effectiveness of this program, we conducted both a pre-test and post-test with nine participants who completed the experimental module, and we also gathered additional program feedback. The results of the experimental module showed high satisfaction among the participants, who did not encounter significant difficulties in understanding the experimental content. Furthermore, there was a slight increase in interest and career aspirations in molecular biology, and knowledge of intracellular protein roles and related molecular biology significantly improved. Therefore, This experimental program is expected to facilitate students in comprehending molecular biological concepts based on proteins and establishing a foundation for conducting molecular biology-related research, and ultimately to assist in making their informed career choices in the life sciences.
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