ФОРМИРОВАНИЕ ПОЯСКОВ КАСПАРИ В КОРНЯХ ПОЧТИ ИЗОГЕННЫХ ЛИНИЙ ЯЧМЕНЯ, ОТЛИЧАЮЩИХСЯ НАЛИЧИЕМ МЕЛАНИНОВОЙ ОКРАСКИ КОЛОСА

Abstract

The influence of the BLP locus genes, which control the formation of black coloration of the spike glumes and grain pericarp on the formation of Casparian bands in Hordeum vulgare L. plants, was studied. The experiment was carried out with plants of the Bowman variety and three almost isogenic lines obtained on its basis: the BLP line, carrying one recombinant section from the donor of the trait of black coloration of the ear; a line with a dark gray mBLP grain and a line with a light gray gBLP grain, carrying recombinant regions in chromosomes 1H, 2H, 3H. Lignin and suberin deposition were assessed on transverse sections of root tissue. Lignin and suberin on histological sections were stained with an aqueous solution of berberine hemisulfate. On the 7th day after the start of germination, on sections made in the basal part of the root, luminescence with berberine revealed no differences between plants of different genotypes in the level of accumulation of lignin and suberin. On the 10th day, on sections of the basal part of the roots of the gBLP line, staining of the radial walls of the endoderm is noticeable, which indicates the deposition of lignin in the endoderm area, characteristic of the formation of Casparian bands, as well as in the area of metaxylem vessels. Thus, it was revealed that the gBLP genotype is characterized by accelerated formation of apoplastic barriers. The Bowman and gBLP genotypes we selected, which contrast in the rate of formation of Casparian bands, are a convenient object for further identifying the relationship between the rate of formation of Casparian bands and salt tolerance of plants.