Аналіз фосфоліпідних біомаркерів як інструмент для оцінки структури мікробних угрупувань на забруднених радіонуклідами територіях

Abstract

The presence of specific components in the environment can change the state of cenoses. The effect of ionizing radiation on plant communities, animals and humans have been well studied, while the effect on soil microflora has not been insufficiently studied. In this paper investigated the effect of radionuclide contamination on microflora of soil samples from the territory of PTLRW "Red Forest 1" (trench), "Red Forest 2" (outside the trench), " Cooling Pond " and " Zalissia ". Phospholipid fatty acid (PLFA) content was obtained by PLFA analysis. The highest level of total PLFA was observed in the territory of "Zalissia" which was 17.40 ± 10.59 μg / h. From the territory of PTLRW "Red Forest 1" (trench) and "Red Forest 2" (outside the trench) the level of total PLFA was 16.29 ± 3.43 μg / g and 16.40 ± 2.90 μg / h, respectively. The PLFA content of the "Cooling Pond" was significantly different from the "Red Forest" and " Zalissia ". The taxonomic groups assessment of the samples, a fungus, gram-positive and gram-negative bacteria content increased in PTLRW "Red Forest" relative to the point "Zalissia ".