Оценка информативности морфологических изменений эритроцитов при гипервитаминозе А и выбор прогностических признаков его возникновения

Abstract

Introduction. Morphometric methods allowing quantitative estimation of changes in a variety of parameters of studied histological objects are often used to evaluate the results of experimental research. The selection of a more limited number of parameters characterized by diagnostically significant evidence of these changes is based on their informative value. The aims of the study were to evaluate the informative value of changes in morpho-densitometric parameters of erythrocytes under hypervitaminosis A and select signs to predict its clinical occurrence. Materials and methods. We used male Wistar rats randomized into two groups of 9 animals each. Hypervitaminosis A was induced by oral daily administration of an oily solution of retinol palmitate. The animals of the control group received an oil base. The morphodensitometric parameters of erythrocytes were measured in blood smears. We compared and evaluated the informativeness of differences in both mean values and t frequency distributions of the parameters in the samples. Results. Erythrocyte morphodensitometric parameters altered with retinol palmitate administration. The values of a number of measured parameters differed significantly from those in the control even before the onset of signs of hypervitaminosis A. We identified a list of informative parameters and selected prognostic criteria for retinol palmitate overdose, namely reduced erythrocyte surface area and increased curvature of the descending torus. A more limited number of diagnostically significant signs were obtained when assessing the informative value of differences in the frequency distributions of morphodensitometric parameters. Conclusion. In morphometric studies, in addition to estimating the informativeness of differences in averaged values, that of differences in their frequency distribution can be used, thus limiting the number of parameters measured under similar experimental conditions. Keywords: hypervitaminosis A, erythrocytes, morphodensitometry, assessment of informativeness