Иммунофенотипическая характеристика моноцитов периферической крови пациентов с болезнью Альцгеймера и синдромом мягкого когнитивного снижения

Abstract

Aim : to study the features of subpopulation composition of peripheral blood monocytes in patients with Alzheimer's disease (AD) and with amnesic mild cognitive impairment syndrome (aMCI). Material and methods : 20 AD patients aged 73.8 ± 6.8 years, and 10 patients with aMCI aged 61.8 ± 9.6 years enrolled in the study were examined. 16 mentally and somatically healthy age- and gender-matched persons were examined as controls. The peripheral venous blood of patients and controls was the material for the study. The relative number of monocytes was determined with the help of FC-500 Flow Суtofluorometer [Beckman Coulter, USA] using the same settings for all samples. Results : the study revealed a significant decrease of the relative number of classical subpopulations (p < 0.01) and an increase of the proinflammatory monocytes level (p < 0.01) in the group of AD patients compared with the control group. The severity of changes of the monocytes subpopulation composition depended on the dementia severity. The sharpest change of the monocytes composition compared to controls was typical for patients with moderate dementia (p < 0.001). An increase of the subpopulation level of proinflammatory monocytes was also revealed in patients with aMCI (p < 0.05). Conclusion : the study results have revealed the redistribution of monocyte subpopulations in AD and aMCI patients: the decrease in the classical monocytes subpopulation on the background of the increase of the monocytes subpopulation with proinflammatory activity.