Сравнение точности метода абсолютной градуировки и модифицированного метода последовательных стандартных добавок на примере определения 3-(2,2,2-триметилгидразиний)- пропионовой кислоты в моче в условиях нелинейности детектирования (электроспрей)

Abstract

Contemporary determining of pharmaceuticals in biological liquids for the pur­poses of phar­maco­kine­tic studies and therapeutic drug monitoring implies the use of high-per­formance liquid chromatography coupled with mass spectrometry (LC-MS) with electrospray as a method of ionization. However, this techni­que is cha­rac­terized by a rather narrow linear detection range. It causes the restric­tions in quan­titation of analytes by method of absolute calibration. On the example of the determination of 3-(2,2,2-trimethylhydrazinium) propio­nate (THP) in urine it is shown that the method of successive standard additi­ons provides more precise results comparing with those obtained with non-linear absolu­te calib­ration at the same levels of analyte concentrations. Its principal fea­ture is the need to extrapolate the results to an infinite value of ad­dition. Keywords : 3-(2,2,2-trimethylhydrazinium) propionate, LC-MS, detection non­­­linearity, successive standard addition, non-linear absolute calibration (Russian) DOI: http://dx.doi.org/10.15826/analitika.2013.17.2.008 Т atiana Е . Morozova 1 , Georgy V. Karakashev 1 , Pavel N. Sorokoumov 1 , Elena I. Savelieva 1 , Igor G. Zenkevich 2 1 Research Institute of Hygiene, Occupational Pathology and Human Ecology , Russian Federation , Leningrad Region., Vsevolozhsk district, town Kuzmolovsky, Art. Kapitolovo 2 St. Petersburg State University, Russian Federation , Saint-Petersburg