Abstract
The behavior of isolated neural cells of newborn rats in culture after hypothermic storage in sucrose-based solution and saline medium DMEM/F-12 in the presence of serum or brain and liver extracts was studied. Hypothermic storage in saline medium DMEM/F-12 in the presence of serum or liver and brain extracts and sucrose-based solution without serum or in the presence of brain extract allows to preserve the differentiated and stem/progenitor cells populations for 2 days. The hypothermic storage of cells for more than this period results in their death. During the hypothermic storage in DMEM/F-12 the brain/liver extracts together with the serum augment the survival rate for newborn rats neural cells, and under hypothermic storage in sucrose-based solution the brain and liver extracts cause positive and negative effects, respectively, on neural cell survival. It was found that the brain and liver extracts allow to effectively substitute the serum during the hypothermic storage of neural cells in DMEM/F-12 medium.Probl Cryobiol Cryomed 2015; 25(1): 13-23.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.