ОЦЕНКА КУЛЬТУРОМА ОТДЕЛЯЕМОГО ВЕРХНИХ ДЫХАТЕЛЬНЫХ ПУТЕЙ И СОДЕРЖИМОГО ТОЛСТОЙ КИШКИ У ПАЦИЕНТОВ С АТОПИЧЕСКИМ ДЕРМАТИТОМ

Abstract

Aim. The aim of the study is to study the species diversity of the microflora of the discharge of the nose, oropharynx and the contents of the colon in atopic dermatitis. Material and methods. The study included 80 patients with atopic dermatitis. Patients are divided into 3 groups depending on the severity of AD (Focusing on the SCORAD scale). The patients underwent collection of biomaterials from the oral and nasal cavities and intestines. Sowing of the material was carried out on an extended list of nutrient media. As a result of the MALDI-ToF mass spectrometry on the Microflex LT (Bruker, Germany), the resulting microorganisms were recognized by direct and extended deposition using formic acid. Results and discussion. When seeding feces patients from all groups, statistically significant differences were revealed for the following microorganisms, depending on the severity: Enterococcus faecium (61%), Streptococcus anginosus (16.7%), Parabacteroides distasonis (22.2%) were found in remission; at the stage of exacerbation with exacerbation of atopic dermatitis in the form of a limited form - Enterococcus faecalis (39.3%), Lactobacillus fermentum (16.1%), Streptococcus parasanguinis (9%);with a common form of exacerbation, Klebsiella pneumonia (50%), Klebsiella oxytoca (50%), Enterococcus mundtii (16.7%), Echerichia vulneris (16.7%), Lactobacillus salivarius (83.3%), Raoultella ornithinolytica(16.7%), Enterococcus avium (50%), Enterobacter asburie (16.7%), Citr obacter braaki (33.3%), Bacteroides vulgates (33.3%), Bifidobacterium adolescentis(16.7%), Enterococcus durans (16.7%), Lactobacillus crispatus (16.7%), Corynebacterium amycolatum (33.3%), Streptococcus sanguinis (16.7%). Inoculation of oropharyngeal discharge from patients with atopic dermatitis revealed statistically significant differences for the following microorganisms, depending on the severity: Streptococcus australis (11.1%) was detected in remission; in the stage of exacerbation with a limited form of atopic dermatitis, Rothia mucalaginosa (19.6%), Streptococcus saliverius (39.3%) were identified; in the stage of exacerbation with a widespread form of atopic dermatitis, Streptococcus anginosus (16.7%;), Candida albicans (33.3%), Streptococcus gordonii (16.7%), Staphylococcus haemolyticus (16.7%), Neisseria oralis (33.3%), Corynebacterium amycolatum (16.7%), Kocuria rhizophila (33.3%), Lactobacillus rhamnosus (16.7%). Inoculation of nasal discharge from patients with atopic dermatitis revealed statistically significant differences for the following microorganisms, depending on the severity: Staphylococcus haemolyticus (16.7%), Staphylococcus lugdunensis (11.1%), Staphylococcus copitis (11.1%) were found in remission. At the stage of exacerbation with a widespread form of atopic dermatitis, Proteus mirabilis (16.7%), Streptococcus vestibularis (16.7%), Streptococcus sobrinus (16.7%), Staphylococcus warneri (16.7%), Corynebacterium coyleae (16.7%), Lactobacillus plantarum (16.7%). Conclusion. Our results indicate enzymatic deficiency and significant changes in the qualitative composition of the microbiota in people with atopic dermatitis, which violates the body's immunomodulating function.