Abstract

The chemical composition of 21 accessions of Lolium perenne L. from the collection nursery of Institute of Forage Crops - Pleven, for three cuts each year, for the period 2017-2018, was evaluated as follows: crude protein (CP), % – according to Kjeldahl‘s method; crude fiber (CF), % – by Weende analysis; water-soluble carbohydrates (WSC) – according to Ermakov et al. Statistical processing of the data was performed, which included regression, correlation, two-factor ANOVA a nd cluster a nalyses. There is a t rend i n both years for the first cut to be lowest in crude protein (CP); relatively high in crude fiber (CF) and highest in water soluble carbohydrates (WSC) content. In the second and third cuts, crude protein increases; crude fiber content varies within different limits, but generally decreases; and the amount of water-soluble carbohydrates also decreases. The genotype–environment (accessions x cuts) interaction had insignificant variance in all three studied traits. In terms of CP, the maximum value was reported for the Iljo variety (18.8%) and the minimum for China (14.6%). No significant differences were observed between the check Tetrany (16.1%) and the other accessions. Only two cases of significant differences were observed for CF and WSC between accessions. The cluster analysis arranges the accessions into three groups, with the second cluster of 8 accessions being the most interesting for the selection. The analysis well differentiates the accessions regarding the optimal combination of the three traits in one genotype, as well as their ploidy level. A significant negative correlation was observed between crude protein and crude fiber content (r=-0.744).

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