Влияние однонуклеотидных полиморфизмов генов свертывающей системы крови и фолатного цикла на тяжесть течения и исходы COVID-19

Abstract

Objective. To characterize the relationship of alleles of single nucleotide genetic polymorphisms in the genes of the hemostasis system and folate cycle with the severity of the course and outcomes of the COVID-19 coronavirus infection. Patients and methods. The study conducted on the basis of the Republican Clinical Infectious Diseases Hospital of Izhevsk in 2020–2021 included 40 patients with COVID-19. The study of alleles of single nucleotide polymorphisms (SNP) of 12 genes encoding factors of the blood coagulation system was performed in patients. Biological analysis of genomic DNA was performed using a set of reagents "Real-Best-Genetics Hemostasis (12)". A CFX96 amplifier (Bio-Rad, USA) was used to carry out the polymerase chain reaction. The significance level of differences between the groups was determined using the criteria χ2, Mann–Whitney and Fisher's exact criterion. Results. The C allele of SNP of ITGB3:1565 β3 integrin gene (T/C) tends to be associated with a more severe course of the disease (p = 0.048). The C allele of SNP of β3 integrin gene ITGB3:1565 (T/C), the T allele of SNP of α2 integrin gene ITGA2:807 (C/T), and the T allele of SNP of methylenetetrahydrofolate reductase gene MTHFR:677 (C/T) tend to be associated with higher mortality with COVID-19 (p < 0.05). Conclusion. The minor allele of SNP of ITGB3:1565 (T/C) gene can be considered as a predictor of the severe course of COVID-19, minor alleles of SNP of ITGB3:1565 (T/C), ITGA2:807 (C/T), and MTHFR:677 (C/T) genes can be considered as predictors of a higher risk of a lethal outcome. Key words: COVID-19, single nucleotide gene polymorphisms, blood coagulation system, folate cycle