Abstract

Використання поліетиленоксиду і гідроксіетилкрохмалю у якості замінника сироватки крові при кріоконсервуванні клітин інтерстицію сім’яника мишей

Highlights

  • The substitution of synthetic polymers such as polyethylene oxide (PEO) and hydroxyethyl starch is of great importance for the development of cryopreservation techniques

  • The research aim was to investigate the effect of polymers, namely PEO and HES in concentration of mg/ml on the survival of mouse interstitial cells (ICs) during cryopreservation with . ; . and

  • We examined preservation of Leydig cells by histochemical staining for β-hydroxysteroid dehydrogenase (HSD) activity

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Summary

Introduction

Use of Polyethylene Oxide and Hydroxyethyl Starch as Serum Substitute for Cryopreservation of Mouse Testis Cells I.O. Kostina1, O.V. Pakhomov1,2 Cryopreserved testicular cells can be used to study testicular cell defects, to preserve endangered species, as well as in reproductive technology. The substitution of synthetic polymers such as polyethylene oxide (PEO) and hydroxyethyl starch is of great importance for the development of cryopreservation techniques. The research aim was to investigate the effect of polymers, namely PEO ( kDa) and HES ( kDa) in concentration of mg/ml on the survival of mouse interstitial cells (ICs) during cryopreservation with .

Results
Conclusion

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