Abstract
The application of cryopreserved autologous bone marrow (BM) is the reconstructive therapy tool for autoimmune diseases (AIDs). The BM use efficiency is determined by preservation rate of its hematopoietic and mesenchymal stem cells (HSCs and MSCs, respectively). This fact determines the need to design the optimal cryopreservation regimens for the BM of AIDs donors. Here we assessed a functional status of bone marrow HSCs and MSCs of mice with adjuvant arthritis (AA), initiated by Freund's complete adjuvant administration. The bone marrow of CBA/H mice to day 14 of AA (chronic stage) and that of healthy animals were cryopreserved under dimethyl sulfoxide protection. The content of HSCs of various differentiation extent was determined by spleen colony formation in vivo to days 8 (CFU-S-8) and 14 (CFU-S-14). Functional activity of MSCs in BM was studied in vitro by the content of fibroblast colony forming units (CFU-F). There was established the fact of altering the functional potential of hematopoietic progenitor cells of BM of various differentiation levels (CFU-S-8, CFU-S-14) and their stromal microenvironment (CFU-F) during clinical manifestation of AA. After cryopreservation of AA animals' BM cells with 7% DMSO a functional potential of CFU-S and CFU-F was established to be similar to the healthy animals' BM, cryopreserved with 10% DMSO. The cryopreserved with the proposed regimen autologous BM may be used for a targeted recovery of lymphohemopoietic system in AA animals. Probl Cryobiol Cryomed 2016; 26(1):63-72.
Highlights
The application of cryopreserved autologous bone marrow (BM) is the reconstructive therapy tool for autoimmune diseases (AIDs)
Established in our research redistribution in subpopulations of CFU-S with various differentiation level which accompanied adjuvant arthritis (AA) development could be the evidence of changes in structural and functional characteristics
In our previous studies we have shown that AA resulted in a lack of the most differentiated hematopoietic progenitor cells that could be a possible reason for increased content of more potent CFU-S-14 [6, 20]
Summary
The application of cryopreserved autologous bone marrow (BM) is the reconstructive therapy tool for autoimmune diseases (AIDs). The BM use efficiency is determined by preservation rate of its hematopoietic and mesenchymal stem cells (HSCs and MSCs, respectively). This fact determines the need to design the optimal cryopreservation regimens for the BM of AIDs donors. Возможным подходом «реставрации» иммунной системы больных с АИЗ, в частности, ревматоидным артритом (РА), является аутологичная трансплантация клеток костного мозга (КМ) на фоне высокодозовой химиотерапии [7, 10]. A possible approach of AIDs patient immune system “restoration”, in particular those with rheumatoid arthritis (RA), is an autologous transplantation of bone marrow (BM) cells together with a high-dose chemotherapy [3, 16]. Полученные результаты свидетельствуют о нарушении взаиморегулирующей активности субстратов лимфогемопоэтической системы в условиях развития АИЗ
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