Метаболические изменения в эндолимфе при профилактическом и лечебном применении производного триазино-индола на модели акустической травмы

Abstract

Objective of the study. To investigate the effect of triazino-indole derivative on the parameters of glycolysis in the endolymph during preventive and therapeutic use on the model of acoustic trauma. Experimental animals and methods. The experiments were carried out on 82 mice, female F1 hybrids of the CBA and C57BL/6 lines weighing at least 17 g at the age of 4–12 weeks. When simulating an acute acoustic trauma, an animal was exposed to white noise with a level of 107 dB SPL and frequency band 3–100,000 kHz. For therapeutic purposes, a single intravenous injection of a triazino-indole derivative was carried out immediately after noise exposure. The drug was administered at doses of 5, 7, and 10 mg/kg of animal weight. For prophylactic purposes, the intramuscular drug injection was administered before the acoustic trauma application. The drug was administered at doses of 5, 7, and 10 mg/kg of animal weight once a day for 3 days before noise exposure. The Cytoflavin was a reference drug. A normal saline was administered to the control group. When using the acoustic trauma models, the metabolic processes level in the cochlea was assessed by the glucose and lactic acid (lactate) content in the labyrinth fluid before and 2 hours after pathological exposure. Results and discussion. The triazino-indole derivative, both during preventive and therapeutic administration, significantly corrects changes in the glycolysis (glucose, lactate) indicators in the endolymph, thereby improving the bioenergetics of the inner ear cells. The best effect is observed when the drug is administered at a dose of 10 mg/kg. When compared with Cytoflavin, the antihypoxic effect of the triazino-indole derivative is more pronounced.