ГЕНЕРАЦІЯ АКТИВНИХ ФОРМ КИСНЮ ЛЕЙКОЦИТАМИ КРОВІ ЩУРІВ ПІСЛЯ ІМПЛАНТАЦІЇ ХІРУРГІЧНОЇ СІТКИ З ПОКРИТТЯМ НА ОСНОВІ ТАНТАЛУ

Abstract

Introduction. Over the past 20 years, polypropylene has become the main material used in hernioplasty, and has proven itself as an excellent material for the restoration of the abdominal wall during hernia repair. Since polypropylene surgical meshes do not decompose well in the body, they can stimulate the development of an inflammatory process in the surrounding tissues, which subsequently causes adhesions. The development of a postoperative inflammatory process after implantation of polypropylene surgical meshes is observed in 30–40 % of patients. This affects the management of the postoperative period, increases the time of stay of patients in the hospital and their period of convalescence. These data force scientists to continue the search for the optimal surgical mesh, which would suit specialists not only from the side of the physical properties of the surgical mesh, but also from the side of its biocompatible and anti-inflammatory properties. Tantalum is successfully used to produce biocompatible medical implants in surgery, orthopedics and dentistry. In previous studies, we have repeatedly noted its excellent anti-inflammatory and antibacterial properties, indicating the possibility of its use as a coating for mesh implants. One of the typical responses to surgical intervention is the generation of reactive oxygen species by leukocyte neutrophils, which are signaling molecules that damage the endothelium of vessels and promote the migration of cells of the immune system to the center of inflammation. The aim of the study – to determine the generation of reactive oxygen species in leukocytes of rats of the control group and experimental rats with implantation of uncoated and tantalum-based surgical meshes. Materials and Methods. ROS generation was assessed in rat blood leukocytes using the dye 2,7-dichlo­ro­dihydrofluorescein diacetate (H2DCFDA) by flow cytometry 28 days after implantation of uncoated and tantalum-coated surgical meshes. Results and Discussion. Analyzing the obtained results, it was determined that the use of tantalum-based surgical meshes does not cause excessive generation of ROS by leukocytes, in contrast to the use of an implant without a coating. Implantation of uncoated surgical mesh caused excessive production of reactive oxygen species in blood leukocytes of rats, as evidenced by statistically significant differences in the mean fluorescence intensity of 2,7- dichlorodihydrofluorescein diacetate. Conclusions. The use of tantalum-based surgical meshes causes less generation of ROS in leukocytes compared to the use of uncoated surgical meshes, and does not provoke the development of adhesions and purulent-septic processes in the postoperative period, which is confirmed by a morphological study. This determines the possibility of their use in surgical practice to improve the durability and stability of use as biomedical implants and prevention of adhesion formation.