Нетозобразующая способность нейтрофилов у детей с латентной туберкулезной инфекцией при положительной пробе с аллергеном туберкулезным рекомбинантным

Abstract

Publications on the role of neutrophils in tuberculosis are contradictory. The potentially versatile role of neutrophils in the pathogenesis of tuberculosis confirms that the participation of granulocytes in tuberculosis infection is important. Patients and methods. The prospective single-center study included 24 patients with latent tuberculosis infection at the time of the first positive test for the tuberculosis recombinant allergen in the absence of radiological changes and 29 children who made up the control group according to the results of the annual tuberculin diagnostics, with a negative reaction to the tuberculosis allergen with 2TE. In the formed groups, the netosis-forming ability of an isolated fraction of neutrophils of peripheral venous blood was determined in vitro using an original technique developed by members of the author's team Results. Neutrophils of children in the study groups demonstrated different levels of netosis-forming ability, and in the group with latent tuberculosis infection, the non-forming ability of neutrophils was higher in comparison with the control group. Neutrophils of both groups formed mainly filamentous traps. In the preparation of a group of children with latent tuberculosis infection, after stimulation of granulocytes with a probiotic, there was a decrease (p = 0.0011) in the percentage of hypoactivated neutrophils relative to the control group. Conclusions. Changes in the proinflammatory and antimicrobial properties of neutrophils can become one of the markers that allow us to assess the dynamics of localization of tuberculosis. Key words: children, latent tuberculosis infection, neutrophil extracellular traps, neutrophil non-forming ability, tuberculosis