Abstract
An acute foot-shock stress caused a reduction in parenchymal-stromal ratio due to the dilation and hyperemia of the liver blood vessels, a stagnation phenomenon in the bile capillaries and cholestasis, an increase in the intensity of the liver current functioning and development of degenerative and destructive changes in hepatocytes, an increase in the current proliferative activity of hepatocytes along with the reduced depth of reparative reserves according to the results of the morphometric examination of the liver. Delta sleep-inducing peptide (DSIP) in the every dose used (40, 120, and 360 mcg/kg) increased the parenchymal-stromal ratio indicators in stressed animals. Peptide introduction in a dose of 40 mcg/kg was accompanied by increasing the current rate of functioning hepatocytes and accumulating the potential reparative reserves in stressed animals. DSIP in a dose of 360 mcg/kg contributed to a decrease in hyperemia, stagnation phenomenon and cholestasis, a significant reduction in degenerative processes and an increase in the liver reparative reserves. In conditions of the acute foot-shock stress the thymus relative weight was also reduced, but it was leveled after administrating DSIP in doses of 40 and 360 mcg/kg. Thus both the stress and peptide administration had no effect on the relative liver weight.
Highlights
Belykh A.E.1,3, Dudka V.T.2, Bobyntsev I.I.1,3, Kryukov A.A.1 1 Department of Pathophysiology, 2 Department of Pathological Anatomy, 3 Research Institute of General Pathology of Kursk State Medical University, Kursk An acute foot-shock stress caused a reduction in parenchymal-stromal ratio due to the dilation and hyperemia of the liver blood vessels, a stagnation phenomenon in the bile capillaries and cholestasis, an increase in the intensity of the liver current functioning and development of degenerative and destructive changes in hepatocytes, an increase in the current proliferative activity of hepatocytes along with the reduced depth of reparative reserves according to the results of the morphometric examination of the liver
Peptide introduction in a dose of 40 mcg/kg was accompanied by increasing the current rate of functioning hepatocytes and accumulating the potential reparative reserves in stressed animals
Delta sleep-inducing peptide (DSIP) in a dose of 360 mcg/kg contributed to a decrease in hyperemia, stagnation phenomenon and cholestasis, a significant reduction in degenerative processes and an increase in the liver reparative reserves
Summary
Эксперименты выполнены на 50 крысах-самцах Вистар массой 310-340 г, разделенных на группы по 10 животных, которые содержались в стандартных условиях вивария со свободным доступом к воде и пище при 12-часовом световом режиме и контролируемой температуре (22±2°С). Пептид растворяли в физиологическом растворе и вводили внутрибрюшинно за 60 мин до начала каждого стрессорного воздействия в дозах 40, 120 и 360 мкг/кг массы тела. Контрольным животным вводили эквивалентные объемы физиологического раствора из расчета 1 мл на 1 кг массы тела. Интактные животные не получали стрессорного воздействия, на протяжении эксперимента им вводили объемы физиологического раствора, эквивалентные таковым у контрольных стрессированных крыс. Для этого рассчитывали отношение массы органа к массе тела соответствующего животного и умножали полученный результат на 1000. После выведения животных из эксперимента для морфологического исследования атравматично забирали печень и вилочковую железу. Для фиксации кусочков изучаемых органов использовали формалин 10% в фосфатном буфере, затем проводили заливку тканей в парафин, готовили гистологические срезы толщиной 6-7 мкм и окрашивали гематоксилином и эозином.
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