Сочетание стрессовых воздействий в критические периоды развития повышает устойчивость к стрессу воспалительной боли у взрослых крыс

Abstract

We studied the long-term effect of the combination of stress in the prenatal and prepubertal periods of development on indicators of tonic inflammatory pain in the formalin test and the depressive-like behavior, as well as on the stress reactivity of the hormonal response in adult rats. In addition, in rats of both sexes, the effect of serotonin reuptake inhibitor (5-HT) fluoxetine and 5-HT1A agonist buspirone, chronically administered to their stressed mothers during pregnancy, on the studied types of adaptive behavior disturbed by prenatal stress was evaluated. It was found that in rats of both sexes, prenatal stress intensified the pain response organized at the spinal and supraspinal levels of the central nervous system, fluoxetine and buspirone normalized them. Stress in the prepubertal period of development leveled the effect of prenatal stress on the inflammatory pain response integrated at the supraspinal level in adult rats; under these conditions, fluoxetine and buspirone did not act, unlike their antinociceptive effect on the pain response integrated at the spinal level. Stress in prepubertal age leveled the sex differences found in the depressive-like behavior in prenatally non-stressed and prenatally stressed rats with saline. In control adult females and adult females with prenatal effects, prepubertal stress increased plasma corticosterone after forced swimming compared to basal hormone levels, but no significant differences in the level of stress reactivity of the hormonal response after forced swimming were found. Thus, the conditions of stressful impacts that increase resistance to stress in adult rats are identified. Stress in critical periods of development forms a phenotype with increased stress resistance to inflammatory pain, which was found in the response organized at the supraspinal level in adults.