ความหลากหลายทางพันธุกรรมของพลับพลึงธาร พันธุ์ไม้น้ำใกล้สูญพันธุ์ในประเทศไทย และพืชในวงศ์ Amaryllidaceae โดยอาศัยเครื่องหมายโมเลกุลจำเพาะกับยีน Genetic variation of the endangered species, water-onion (Crinum thaianum) in Thailand and plants in family Amar

Abstract

Genetic variation among 77 samples of the endangered species, water-onion ( Crinum thaianum ) in Thailand and five plants in family Amaryllidaceae, including C. asiaticum , Hymenocallis littoralis , C. amabile , C. nathans and Hymenocallis sp . , was studied using PCR-RFLP markers specific to seven genes, NADH dehydrogenase subunit F ( ndh F), Acetyl-CoA carboxylase beta subunit ( acc D), NADH-plastiquinone oxidoreductase subunit 7 ( ndh H), NADH-plastoquinone oxidoreductase subunit 1 ( ndh A), Photosystem I P700 apoprotein A2 ( psa B), 18S rDNA and 26S rDNA. All samples could be separated into two main groups and 4 subgroups. Group I consisted of two subgroups, one was all water-onion samples and the other was C. asiaticum and C. amabile . Group II also consisted of two subgroups, one was Hymenocallis sp. and the other was H. littoralis and C. nathans . The developed PCR-RFLP markers could not distinguish all studied water-onion samples. Other DNA markers with high polymorphism and multi-locus detection should be used to study genetic variation among water-onion samples. Partial sequences of seven genes of water-onion were analyzed in order to confirm the gene specificity and for the specific identification of Thailand water-onion. Five partial gene sequences ( acc D, ndh H , ndh A , psa B, 26S rDNA) of water-onion were compared to other plant sequences available in database. The result indicated that water-onion was most closely related to C. asiaticum . This first report on water-onion gene sequences will be useful for the development of suitable DNA markers to evaluate water-onion genetic information and for conservation and sustainable use of water-onion in the future.