Динамика метаболических показателей у крыс на разных стадиях постстрессорного периода в условиях антигенного воздействия при введении липополисахарида

Abstract

The dynamics of parameters for the intensity of metabolic processes in rats was studied after intraperitoneal injection of lipopolysaccharide (LPS, 100 µg/kg) by the end of a single exposure to long-term stress on the model of 24-h immobilization. The volumes of consumed oxygen and expired carbon dioxide, as well as the level of heat emission, were evaluated on a Phenomaster automated module device (TSE Systems GmbH, Germany) 3 h and 1 or 8 days after the treatment. Study parameters of metabolism in animals of the physiological saline group were shown to increase significantly 1 day after restraint stress. The observed variations illustrate intensification of metabolic processes in rats during the early period after experimental stress. These changes persisted even in the delayed stage after stress load (day 8 of observations). The specimens receiving LPS were characterized by a rise in oxygen consumption, carbon dioxide expiration, and level of heat exchange on day 1 of the post-stress period. On day 8 after stress load with the subsequent immune modulation, metabolic parameters in rats were revealed to decrease and did not differ from those in intact animals. Therefore, the antigenic treatment by systemic administration of LPS has no effect on a stress-induced activation of metabolic processes at the early stage of observations, but prevents a persistent increase in the intensity of metabolism during the delayed post-stress period. Our results and published data suggest that the influence of LPS, which possesses immunoactive properties, is related to the modulatory effect on neuroendocrine mechanisms for regulation of homeostasis in mammals, e.g., under stress conditions.