Abstract
The aim of the present work was to develop an approach for producing tissue engineered constructs based on mesenchymal stromal cell culture and new-generation biodegradable synthetic polymer matrices obtained by the technology of surface-selective laser sintering. Primary mesenchymal stromal cells were derived from the pulp of naturally shed deciduous teeth. Following seeding, the cells were cultured on matrices for ten days. Cell viability was quantitatively assessed using CytoTox Assay (Promega) based on the colorimetric measurement of mitochondrial dehydrogenase activity. Cellular structure and spatial arrangement within a tissue-engineered matrix was visualized by scanning electron microscopy (SEM), using supravital lanthanoid staining. The results obtained showed that matrices under development enable good cell adhesion and proliferation, and hence can be used as a cell carrier in tissue engineering. Thus, the techniques of surface-selective laser sintering, which was developed by us, could find wide use in regenerative medicine.
Highlights
The aim of the present work was to develop an approach for producing tissue engineered constructs based on mesenchymal stromal cell culture and new-generation biodegradable synthetic polymer matrices obtained by the technology of surfaceselective laser sintering
Primary mesenchymal stromal cells were derived from the pulp of naturally shed deciduous teeth
Cellular structure and spatial arrangement within a tissue-engineered matrix was visualized by scanning electron microscopy (SEM), using supravital lanthanoid staining
Summary
The aim of the present work was to develop an approach for producing tissue engineered constructs based on mesenchymal stromal cell culture and new-generation biodegradable synthetic polymer matrices obtained by the technology of surfaceselective laser sintering. Оценку количества жизнеспособных клеток проводили при помощи набора CytoTox Assay (Promega), основанного на колориметрическом измерении активности митохондриальных дегидрогеназ. Для визуализации клеток в составе тканеинженерных конструкций применяли сканирующую электронную микроскопию (СЭМ) с суправитальным лантаноидным контрастированием.
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