Abstract
Ðа модели адъювантного артрита у мышей линии СВÐ/РиÑÑледованы гематологичеÑкие показатели, определен уровень циркулирующих иммунных комплекÑов (ЦИК) и количеÑтво Ñтволовых кроветворных клеток (CD34+CD38–, CD117+) в коÑтном мозге (КМ) до и поÑле Ð¿Ñ€Ð¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð½Ð°Ñ‚Ð¸Ð²Ð½Ð¾Ð¹ и криоконÑервированной в различных режимах ÑуÑпензии клеток плаценты (СКП). СКП криоконÑервировали Ñ 10% диметилÑульфокÑида или 10% пропандиоÑахаролÑ. МакÑимальное терапевтичеÑкое дейÑтвие оказывало введение нативной СКП (14-е Ñутки) и ÑуÑпензии, криоконÑервированной Ñ Ð¿Ñ€Ð¾Ð¿Ð°Ð½Ð´Ð¸Ð¾Ñахаролем (28-е Ñутки). Введение любой из ÑуÑпензий корригировало ÑкороÑÑ‚ÑŒ оÑÐµÐ´Ð°Ð½Ð¸Ñ Ñритроцитов, ЦИК, количеÑтво ÑдроÑодержащих клеток в КМ, но Ñффект проÑвлÑлÑÑ Ð² различные Ñроки. Применение криоконÑервированных ÑуÑпензий ÑпоÑобÑтвовало воÑÑтановлению индекÑа пролиферативной активноÑти Ñтволовых кроветворных клеток. Ð’ завиÑимоÑти от уÑловий криоконÑÐµÑ€Ð²Ð¸Ñ€Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¡ÐšÐŸ изменÑлиÑÑŒ ÑвойÑтва вводимой ÑуÑпензии, что влиÑло на Ñроки воÑÑÑ‚Ð°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸Ñ‡ÐµÑтва CD117+-клеток. Probl Cryobiol Cryomed 2019; 29(1): XXX–XXX
Highlights
In the model of adjuvant-induced arthritis in CBA/H mice there were investigated the gematological indices, circulating immune complexes (CIC) in peripheral blood, a number of hematopoietic stem cells: CD34+CD38– and CD117+ in bone marrow (BM) before and after applying either fresh or cryopreserved with various regimens placental cell suspension (PCS)
It has been established earlier that in rare complications of rheumatoid arthritis (RA) (Felty’s syndrome), the observed similar neutropenia may result from death of neutrophils loaded with antibodies or immune complexes and phagocytosis [14, 19]
We have proven that when changing the parameters of cryopreservation, in particular using different cryoprotectants, it was possible to inhibit the function of accessoryregulatory elements in BM, thereby reducing an immune reactivity of myelograft in the graftversus-host disease [3]
Summary
In the model of adjuvant-induced arthritis in CBA/H mice there were investigated the gematological indices, circulating immune complexes (CIC) in peripheral blood, a number of hematopoietic stem cells: CD34+CD38– and CD117+ in bone marrow (BM) before and after applying either fresh or cryopreserved with various regimens placental cell suspension (PCS). The maximum therapeutic effect was found after the introduction of fresh PCS (day 14) and suspension, cryopreserved with propane diol saccharol (day 28). Autoimmune diseases, including rheumatoid arthritis (RA), are accompanied with the significant impairments in the function of hematopoietic system Patients suffering from this pathology usually have a decreased number of hematopoietic progenitors, enhanced apoptosis of CD34+ cells in bone marrow (BM), changes in the clonogenic.
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