КОГНИТИВНЫЙ И НЕВРОЛОГИЧЕСКИЙ СТАТУС КРЫС ПОСЛЕ МОДЕЛИРОВАНИЯ ОСТРОЙ ЧЕРЕПНО-МОЗГОВОЙ ТРАВМЫ НА ФОНЕ ПРИМЕНЕНИЯ РАЗЛИЧНЫХ КОНЦЕНТРАЦИЙ КСЕНОНА

Abstract

The study aims to evaluate the efficacy of xenon inhalation at various concentrations on neurological and cognitive impairments when simulating open traumatic brain injury (TBI) in rats. Tests were performed on 35 male Wistar rats weighing from 250 to 350 g. After simulating an open traumatic brain injury, the animals received inhalation with a mixture of gases: 5 sham operated animals received only anesthesia without traumatic brain injury and inhalations, a control group with traumatic brain injury + inhalation N2 70 % / O2 30 % (group “TBI”, n = 10); an experimental group with traumatic brain injury + inhalation of xenon-oxygen 70 % / O2 30 % (group “TBI + iXe70”, n = 10), an experimental group with traumatic brain injury + inhalation of xenon-oxygen and nitrogen – Xe 35 % / O2 30 %, nitrogen 35 % (group “TBI + iXe + N2”, n = 10) for 60 minutes. After gas inhalation, the animals were observed for 14 days. On the 3rd, 7th and 14th days, the neurological limb placement test was carried out. The preservation of cognitive functions, such as learning and spatial memory, was assessed using the Morris water maze test with control testing on the 14th day. After testing, on the 14th day of observation, euthanasia was performed, and the brain was collected for research. It was found that the selected xenon concentrations in doses of 0.25 and 0.5 MAC tatistically significantly reduced the severity of both neurological deficit and cognitive impairment in 7 and 14 days, whereas increasing the xenon concentration did not significantly enhance the neuroprotective effect.