Особливості розвитку репродуктивної системи щурів нащадків чоловічої статі, що народжені від батька, який до спаровування отримував фітоестрогени

Abstract

The purpose of the study was the research of the influence of rats father's excessive consumption of phytoestrogens on somato-sexual development and reproductive function of male offspring. Materials and methods. Male parents were fed with a mixture of phytoestrogens before mating with intact females at a dose of 20 mg/kg of body weight for 30 days. In reproductive animals, reproductive function was studied for such characteristics as sexual behavior, fertility, spermogram, the mass of androgen-dependent organs was determined, and the level of sex hormones was determined. Experimental parents were divided into two groups: 1) intact, 2) males who received excess phytoestrogens. After the birth of male offspring from these parents, their reproductive system was examined. Results and discussion. Prolonged administration of phytoestrogens to mature male parents (parents) causes absolute and relative hyperestrogenism (five-fold increase in E2 and vice versa reduction of T/E2 three-fold), which leads to a decrease in copulatory activity (reduction of the number of nurseries and intromissions by 28%), violation of spermatogenesis (reduction of sperm concentration by 39%, doubling the proportion of abnormal forms), at the same time does not affect the reproductive potential of male who were born to fathers who received an excess consumption of phytoestrogens. The presence of excess compounds with estrogenic activity in the diet of adult males in the preconsumption period leads to the birth of male offspring, which have reduced testosterone levels (25%) in the critical period of sexual differentiation of the brain (5 days of life). Descendants of the male sex of the phytoestrogenized father have a normal course of somatic development but are characterized by impaired sexual development (less anogenital distance by 13%) and delayed lowering of the testicles into the scrotum, lower body weight in adulthood (28.2% in 150 day rats). Reproductive function of the offspring of phytoestrogenized father is characterized by differences in sexual behavior: less copulatory activity – 50%, less number intromission, the number of males with ejaculation is 10%; delayed sexual reactions; and lower reproductive potential (two-fold reduction of the integrated indicator of potential fertility of animals). Decreased reproductive potential of the offspring, who were born to fathers who received an excess consumption of phytoestrogens, occurs due to a decrease in the proportion of effective fertilization (pregnant females among those fertilized only 60%), indicating negative changes in sperm, the development of which, starting from the stage of spermatocytes, occurred in conditions of absolute and relative hyperestrogenism. Conclusion. The male who were born to fathers who received an excess consumption of phytoestrogens, have complications in the functioning of the reproductive system