Зміни функціонального стану прооксидантної та антиоксидантної систем у надниркових залозах морських свинок за умов формування експериментального алергічного альвеоліту

Abstract

Introduction. Important role in understanding the pathogenesis of allergic alveolitis is played by processes of lipid peroxidation (LPO) and antioxidant system (AOS). Under physiological conditions, the level of LPO is maintained due to the equilibrium of pro- and antioxidant systems. LPO is considered as a universal mechanism of cell damage at the membrane level in conditions of different pathological conditions. In particular, hypoxia, inflammation, stress, allergy activate lipoperoxidation processes and suppress both an enzymatic and non-enzymatic activity of antioxidant defense. Products of lipoperoxidation negatively affect cell membranes and have a direct damaging effect on the adrenal gland tissue.The aim of the study – to evaluate the influence of lipid peroxidation processes and the state of the antioxidant system in the adrenal glands in the conditions of the formation of experimental allergic alveolitis.Research Methods. The experiments were conducted on 36 guinea pigs, body weight 0.18-0.20 kg. They were divided into four groups (n = 9). Intact – the first group (control). Animals with experimental allergic alveolitis, respectively, on the 1st, 2nd, and 34th day of the experiment. Experimental allergic alveolitis was modeled by O. O. Oriekhov, Yu. A. Kyrylov method. Activity of catalase was studied by B.Holmes, C. Masters method; superoxide dismutase – by R. Fried method; diene conjugates – by V. B. Havrylov, M. I. Mishkorudna method; malonic dialdehyde – by E. N. Korobeinikov method. Statistical processing is standard.Results and Discussion. The results of our studies revealed that the activity of the antioxidant system enzymes varied unidirectionally, depending on the periods of formation of the experimental allergic alveolitis. There is an increase in lipid peroxidation products both in its initial and final stages. At the same time, the activity of individual enzymes of antioxidant system underwent opposite changes.Conclusion. The obtained results indicate significant changes in the state of pro- and antioxidant systems in the thymus of animals with experimental allergic alveolitis and are important for a better understanding of the pathogenesis of experimental allergic alveolitis. These studies provide an opportunity to find more effective and useful ways to correct the detected changes in experimental allergic alveolitis.