Анализ ретинальной экспрессии генов, участвующих в регуляции ангиогенеза и функций эндотелиального барьера после воздействия лазерного излучения с длиной волны 577 нм в непрерывном режиме на сетчатку

Abstract

Objective. The aim of the study was to investigate the retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function after exposure of the retina to 577 nm laser light in a continuous mode in experimental conditions after intravitreal injection of VEGF165 (Vascular endothelial growth factor). Materials and methods. The study was performed on 4-5 week old male C57BL / 6J mice. 4 groups, 5 mice in each, were formed, one animal eye was experimental and the second remained intact. The animals of the first group received intravitreal injection of phosphate-buffered saline (PBS); animals in the second, third and fourth groups received intravitreal injection of 50 ng/ml of recombinant VEGF165; in the third and fourth groups, one day after the VEGF165 intravitreal administration, laser irradiation with a wavelength of 577 nm was performed on the retina in micropulse and continuous modes, respectively. Tissue samples from animals of the first and second groups were taken 2 days after PBS and VEGF165 administration, and in animals from the third and fourth groups one day after laser exposure to the retina. Results. Retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function as a result of retinal exposure to 577 nm laser radiation in continuous mode following intravitreal injection of VEGF165 was studied. Genes with significant changes in expression levels were identified (genes that regulate the processes of angiogenesis). Conclusion. Understanding the mechanisms of modulation of retinal gene expression may help to identify the key regulatory factors providing therapeutic effects of laser radiation in continuous and micropulsed modes and provide the basis for future therapeutic tactics for retinal diseases.