Дослідження залежності тяжкості перебігу екземи рук від деяких показників цитокінового профілю, вмісту вітаміну д та генетичного поліморфізму гена глюкокортикоїдного рецептору

Abstract

Hand eczema is one of the most important chronic diseases in the dermatology. There are a lot of researches presenting the role of individual cytokines in eczema development. However, currently, the complex analysis of cytokines, hormones, and gene polymorphisms' impact on the development of hand eczema and its severity is absent. Therefore, the purpose of our work was to study the possible association between hand eczema severity and rs41423247 SNP, the blood level of IL-17A, IL-2, and 25(OH)2D3. Material and methods. The venous blood of 143 patients with hand eczema aged (42.2±11.1) years was used in the study. During the patients examination the data on age, sex, BMI kg/m2, BMI ≥25 kg/m2 (%), smoking, IgE (iu/ml), IL-17A (pg/ml), IL-2 (pg/ml), and 25(OH)2D3 (ng/ml) were obtained. The HECSI index (Hand Eczema Severity Index) was also assessed in each subject. According to HECSI value all patients were divided into three subgroups: mild, moderate and severe eczema. BclI SNP (rs41423247) of the glucocorticoid receptor gene (NR3C1) was determined using PCR-RFLP method. The statistical analysis was performed using the program SPSS 22.0. Results and discussion. The obtained results showed that the number of overweight people was higher among patients with severe hand eczema compared to patients with mild to moderate disease (P = 0.052). It was also revealed that the concentration of IL-17A in the blood serum of patients with severe hand eczema was significantly higher than in patients with mild to moderate disease (P = 0.037). The distribution of BclI polymorphism genotypes (NR3C1 gene), the blood level of IgE, IL-2, and 25(OH)2D3 were statistically equal between patients with different degrees of hand eczema severity (P > 0.05). Conclusion. Thus, the IL-17A serum concentration and body mass index are related to hand eczema severity. However, the link between NR3C1 gene BclI polymorphism, the blood level of IgE, IL-2, 25(OH)2D3, and hand eczema severity was not detected