Дослідження впливу екстрактів з листя стевії та чорниці на показники розвитку експериментального цукрового діабету, індукованого високофруктозною дієтою з додаванням ін’єкцій дексаметазону

Abstract

Introduction. Diabetes mellitus type 2 (DM 2) is a metabolic disease, which is characterized by chronic hyperglycemia. It develops as a result of insulin secretion disorder or mechanisms of its interaction with tissue cells. It is known that hyperglycemia is the main cause of cardiovascular complications development.The aim of the work – to study the effect of dry extracts from stevia and bilberry leaves on the metabolic disorders development in rats with experimental diabetes mellitus, caused by dexamethozone on a high-fructose diet ground.Methods of the research. The concentration of glycosylated hemoglobin, the level of fructosamine, the area under the glycemic curves, the level of immunoreactive insulin have been determined in the serum. Lipoperoxidation indicators have been determined according to diene conjugates (DC) and TBA-reactive products (TBA-RAP) content, the state of the antioxidant system has been assessed by reduced glutathione concentration determining (GSH) [8]. The significance of intergroup differences have been assessed due to Student's t-criterion.Results and discussion The introduction of low-dose dexamethasone in laboratory animals causes a complex of disorders, which are specific for metabolic syndrome and type 2 diabetes. Thus, a rise in the level of glycosylated hemoglobin, a concentration of fructosamine, a glucose level, a basal glycemia level, an increase in the area under the glycemic curves are observed in the serum of rats under the indicated conditions. With the introduction of dry extracts of stevia and bilberry leaves together with dexamethasone, the normalizing influence of these substances on the investigated indicators is observed.Conclusions. The use of dry extracts from stevia and bilberry leaves has a normalizing effect on glycosylation indicators, glucose level and lipid metabolism in the rats’ serum in type 2 diabetes mellitus. The more pronounced antioxidant effect of the extract from bilberry leaves and the hypoglycemic action of the extract from stevia leaves have been shown. This proves the advisability of the extracts from stevia and bilberry leaves joint application with the aim of drugs creation on their basis for metabolic disorders correction.