You have accessJournal of UrologyCME1 Apr 2023MP14-03 VIRTUAL MONITORING OF BLADDER CANCER PROGRESSION IN MICE WITH INTRAVESICAL CONTRAST ENHANCED MAGNETIC RESONANCE IMAGING (ICE-MRI) Anirban Ganguly, Lesley Foley, T. Kevin Hitchens, Jodi Maranchie, Youko Ikeda, Irina Zabbarova, Anthony Kanai, Naoki Yoshimura, and Pradeep Tyagi Anirban GangulyAnirban Ganguly More articles by this author , Lesley FoleyLesley Foley More articles by this author , T. Kevin HitchensT. Kevin Hitchens More articles by this author , Jodi MaranchieJodi Maranchie More articles by this author , Youko IkedaYouko Ikeda More articles by this author , Irina ZabbarovaIrina Zabbarova More articles by this author , Anthony KanaiAnthony Kanai More articles by this author , Naoki YoshimuraNaoki Yoshimura More articles by this author , and Pradeep TyagiPradeep Tyagi More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003234.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Here, we examined the potential of intravesical contrast enhanced magnetic resonance imaging (ICE-MRI) for virtually monitoring the bladder tumor progression from 2-12 weeks, induced in mice fed water containing tobacco carcinogen, N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) that alkylates DNA to elicit the mutational burden and basal subtype cell markers of muscle invasive bladder cancer (MIBC) as well as reflect the four-fold higher cancer risk in past and current smokers. METHODS: 6 weeks old immunocompetent female mice of B6D2F1 strain (n=20) were fed ad libitum water or water containing 0.05% BBN for upto 12 weeks and imaged by ICE-MRI at 7 T under isoflurane anesthesia, before and after 0.05 mL transurethral instillation of Gadobutrol (4 mM) and Ferumoxytol (5 mM) for a 30 min period. Multi slice, axial T1 weighted images were acquired at variable repetition time of 400-7500 ms for voxel-wise T1 mapping with echo time -6.5 ms, slice thickness 0.8 mm, field of view 28*28 mm2, acquisition matrix 218×218, and number of averages=1. RESULTS: While urinary excretion of Gadobutrol injected i.v. for DCE-MRI generates pseudo layering in lumen, ICE-MRI accomplishes uniform image contrast in lumen with steep concentration gradient that resists dilution from fresh urine for size dependent paracellular diffusion of 0.8 nm sized Gadobutrol while lumen is darkened from retention of 30 nm Ferumoxytol. Paracellular diffusion exclusively of Gadobutrol and not Ferumoxytol across perturbed tight junction complex of cancerous cells shortens T1 relaxation times of water protons for differentiating normal bladder wall (2909 ms) from cancer foci (1048 ms) in color coded T1 maps (attached Figure 1). BBN fed mice exhibit superficial tumor after 2-3 weeks that progresses to MIBC stage (∼4 mm length) at multiple foci by 12 weeks in 50% of BBN mice having bladder wall twice the thickness of water fed mice. CONCLUSIONS: Here, we show that ICE-MRI can visualize small polyps and MIBC owing to tumoritropic infiltration of Gadobutrol. ICE-MRI leverages perturbed tight junction complex of cancer foci relative to normal regions for differential contrast enhancement to perform non-surgical surveillance of indolent cancer growth, detect recurrence and stage MIBC without transurethral resection. Source of Funding: NCI- CA263243, CA251341, P30CA047904 © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e183 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anirban Ganguly More articles by this author Lesley Foley More articles by this author T. Kevin Hitchens More articles by this author Jodi Maranchie More articles by this author Youko Ikeda More articles by this author Irina Zabbarova More articles by this author Anthony Kanai More articles by this author Naoki Yoshimura More articles by this author Pradeep Tyagi More articles by this author Expand All Advertisement PDF downloadLoading ...
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