Red Deer Research Articles

Brucella spp. in Wildlife of the Lombardy Region, Northern Italy.

Surveillance data collected in the period 2017-20 for Brucella spp. in wildlife of the Lombardy Region in northern Italy were used to describe the exposure of the wildlife species to Brucella spp. in wild boar (Sus scrofa), European brown hare (Lepus europaeus), fallow deer (Dama dama), red deer (Cervus elaphus), and roe deer (Capreolus capreolus). Among the tested species, wild boar (n=6,440) showed the highest percentage of seropositive samples (5.9%). Notably, wild boars of perifluvial area of the Po River showed higher percentages of positivity than those of the pre-Alpine district. In addition, during the hunting season in 2018, 95 organs (uterus or testes, spleen, and submandibular lymph nodes) from wild boar of the perifluvial area of the Po River were collected for bacteriological examination. Brucella suis was isolated in culture from 18.9% of tested lymph nodes. These serological and microbiological results highlight the presence of B. suis in wild boar and suggest the importance of wild boar as a reservoir for B. suis. Comparison of the spatial distribution of Brucella-seropositive wild boars with the location of backyard swine farms revealed a higher chance of contact between the two populations only in the areas where the lower percentage of seropositive samples was observed. Conversely, the high percentage of seropositive samples observed in the Po River area coupled with positive microbiological cultures suggest a greater risk of infection for the humans directly or indirectly involved in wild boar hunting activity. These results may serve as a basis to establish sound wildlife management and to adopt education campaigns aimed at reducing the risk of human infection in people involved in wild boar hunting related activities.

PEPPER AND CORN LEAVES CLASSIFICATION AND SEVERITY IDENTIFICATION USING HYBRID OPTIMIZATION BASED U-NET MODEL

Abstract An agricultural product plays a major role in the economical growth of developing countries. Agricultural products like pepper and corn are the essential crops with respect to human health food security. But, these two crops are prone to different diseases such as gray leaf spot, common rust and fruit rot which affects the productivity of crops. Further, the severity identification is also a challenging one. To address these limitations, this work presents different approaches for identifying the crop lesions and predicting the severity and thereby increasing the productivity of crops. The development of the proposed model includes steps such as dataset collection, noise removal, segmentation, feature extraction, classification and severity prediction. Initially, the crop images are pre-processed by the median filter and the pre-processed images are processed are segmented, extracted and classified by the optimized U-Net model. Moreover, hybrid optimizer which is the integration of GJA (Golden jackal algorithm) and RDA (Red deer algorithm) are utilized for precise segmentation and classification. Finally, the severity prediction is computed for the diseased leaves by the measuring the size of image pixels. The experimentation is carried out on the PlantVillage dataset; the accuracy and precision values achieved are 99.2% and 99.1%. Thus, the experimental outcomes show the effectiveness and stability of the model.

Moving towards an ecological management of overabundant ungulates: insights from wildlife-vehicle collisions and hunting bag data

Increasing abundance of large ungulates is raising human-wildlife impacts and the effectiveness of recreational hunting to reduce their population growth is increasingly questioned. We report on long-term trends (> 15 years) in wildlife-vehicle collisions (WVC) and hunting bags, and on associations between the annual growth rate of WVC and that of hunting bags for three ungulates – the wild boar, the red deer, and the roe deer – and the grey wolf in northwest Spain to evaluate the regulating capacity of recreational hunting at large spatial scale. Wildlife-vehicle collisions increased by 332% in 16 years and 91% of all traffic accidents were caused by collisions with these three ungulates. All ungulate species showed significant positive trends in WVC and hunting bags, but we did not observe a negative association between annual growth rate of hunting bags and that of WVC except for the wild boar. Results suggest that recreational hunting was unable to reduce ungulate population growth at the regional scale. There was no upward trend of vehicle collisions with wolves over the study period, possibly reflecting stable wolf populations. Natural mortality due to predation could be promoted through the protection of apex predators, but the lethal management of apex predators, often based on sociopolitical pressures rather than damage levels, can conflict with the strategy for mitigating ungulate impacts. Ungulate management needs to be reconsidered from an ecological perspective that integrates human management measures, including recreational hunting, based on the population dynamics and the recovery of predator–prey interactions by favoring the expansion of apex predators.

Hybridization alters red deer gut microbiome and metabolites

The host genes play a crucial role in shaping the composition and structure of the gut microbiome. Red deer is listed as an endangered species by the International Union for the Conservation of Nature, and its pilose antlers have good medicinal value. Hybridization can lead to heterosis, resulting in increased pilose antler production and growth performance in hybrid deer. However, the role of the gut microbiome in hybrid deer remains largely unknown. In this study, alpha and beta diversity analysis showed that hybridization altered the composition and structure of the gut microbiome of the offspring, with the composition and structure of the hybrid offspring being more similar to those of the paternal parents. Interestingly, the LefSe differential analysis showed that there were some significantly enriched gut microbiome in the paternal parents (such as g_Prevotellaceae UCG-003, f_Bacteroidales RF16 group; Ambiguous_taxa, etc.) and the maternal parents (including g_Alistipes, g_Anaerosporobacter, etc.), which remained significantly enriched in the hybrid offspring. Additionally, the hybrid offspring exhibited a significant advantage over the parental strains, particularly in taxa that can produce short-chain fatty acids, such as g_Prevotellaceae UCG-003, g_Roseburia, g_Succinivibrio, and g_Lachnospiraceae UCG-006. Similar to bacterial transmission, metagenomic analysis showed that some signaling pathways related to pilose antler growth (“Wnt signaling pathway,” “PI3K Akt signaling pathway,” “MAPK signaling pathway”) were also enriched in hybrid red deer after hybridization. Furthermore, metabolomic analysis revealed that compared with the paternal and maternal parents, the hybrid offspring exhibited significant enrichment in metabolites related to “Steroid hormone biosynthesis,” “Tryptophan metabolism,” “Valine, leucine and isoleucine metabolism,” and “Vitamin B metabolism.” Notably, the metagenomic analysis also showed that these metabolic pathways were significantly enriched in hybrid deer. Finally, a correlation analysis between the gut microbiome and metabolites revealed a significant positive correlation between the enriched taxa in hybrid deer, including the Bacteroidales RF16 group, Prevotellaceae, and Succinivibrio, and metabolites, such as 7α-hydroxytestosterone, L-kynurenine, indole, L-isoleucine, and riboflavin. The study contributes valuable data toward understanding the role of the gut microbiome from red deer in hybridization and provides reference data for further screening potential probiotics and performing microbial-assisted breeding that promotes the growth of red deer pilose antlers and bodies, development, and immunity.

Caesium-137 in the muscles of game animals in 2015-2022 – levels and time trend

Abstract Introduction Radioactive caesium-137 occurring in the environment may be taken up by plants and animals and pose a trophic threat to humans. Game animals living in forest ecosystems are very good bioindicators of the level of environmental contamination by ionising radiation. The main species measurably exposed to caesium-137 are the wild boar (Sus scrofa), the roe deer (Capreolus capreolus), and the red deer (Cervus elaphus). The study determined the levels of Cs-137 in muscle samples of these game animals in 2015–2022. Material and Methods Using gamma radiation spectrometry, 858 samples of game animal muscle tissue were examined: 508 wild boar, 145 roe deer and 205 red deer samples. Results Concentrations of Cs-137 varied widely (from minimum detectable activity (MDA) values to over 4,000 Bq/kg). In 63.4% of cases, the obtained concentrations exceeded the MDA. The permissible limit (600 Bq/kg for food) was exceeded in nine wild boar muscle samples, whereas it was not even exceeded once in roe or red deer muscle. The average concentration in wild boar was three times higher than in roe and red deer and amounted to 42.84 Bq/kg. The highest concentration of Cs-137 in wild boar muscle was 4,195 ± 372.0 Bq/kg, in roe deer muscle it was 111.5 ± 12.50 Bq/kg, and in red deer muscle was 86.70 ± 3.470 Bq/kg. Conclusion The seven years’ data indicate that wild boar absorb the most caesium-137 among game animals. The concentrations of Cs-137 in the muscle of game animals in the years 2015-2022 were at a nearly constant level, a very slow diminution being noticeable over time in roe and red deer muscle.

Wild ungulate effects on soil fungal diversity in Mediterranean mixed forests

The effect of wild ungulate density on ecosystems varies according to different factors: climate and physiography conditions, forest type, management history, and herbivore identity. In this study, we evaluated the effect of historically high densities of red deer (Cervus elaphus L.) on the soil fungal communities in Mediterranean ecosystems using 30 paired plots, open on the one hand and exclosure plots on the other one. Plots were established at the end of 2020 in a perimeter-fenced hunting estate of 6600 ha in Toledo, Spain. Three months after plots were established, we analysed fungal communities in 60 soil samples using Illumina 250-bp paired-end sequencing. We estimated changes in total fungal richness and in the richness of trophic groups through Linear Mixed Effects models using fenced/unfenced type and deer habitat use as fixed variables and the location of the plots and the main tree host species as random variables. Fungal composition was analysed using non-metric multidimensional scaling and permutational multivariate ANOVA; edaphic characteristics were incorporated to explain differences. Soil fungal communities were not differentially affected by excluding ungulates for three months. Areas with high deer densities had a richer saprotrophic community and where lowland environments were dominated by the main tree hosts Quercus faginea and Quercus ilex. Arbutus unedo was found in mountain areas where there was less herbivore pressure, a greater richness of ectomycorrhizal and lichenized fungi and soils positively associated to nitrogen, phosphorus, potassium and organic matter levels.

Ungulate niche partitioning and behavioural plasticity of aurochs in Early Holocene southern Scandinavia revealed by stable isotope analysis of bone collagen

This study examines the impact of the extensive climatic and environmental changes associated with the Early Holocene on the feeding ecology of aurochs, European bison, red deer, and Eurasian elk in southern Scandinavia from radiocarbon dates and analysis of stable carbon and nitrogen isotopes. Molecular sex information is utilised in the study of aurochs to understand the underlying reasons behind observed intraspecific differences. Asynchronous diachronic changes in dietary palaeoecology were observed between the included taxa. The observed trends in foraging and habitat use among herbivores in our dataset can be attributed to the directional vegetation change from open landscape to forest. Our findings imply that environmental changes and/or interspecific competition contributed to the local extinction of European bison and impacted the niches of remaining species through niche overlap (cervids) and partitioning (between cervids and aurochs). Thus, our observations are consistent with the influence of multiple mechanisms that acted concurrently.

Helminth Prevalence in European Deer with a Focus on Abomasal Nematodes and the Influence of Livestock Pasture Contact: A Meta-Analysis

Helminth Prevalence in European Deer with a Focus on Abomasal Nematodes and the Influence of Livestock Pasture Contact: A Meta-Analysis

Social and Seasonal Organisation of Red Deer Hunting from the Neolithic to the Late Iron Age (c. 2000 BC to AD 1000)

This is the first paper to examine social and seasonal organization of red deer hunting using stone-set hunting blinds in Norway. The paper examines seven hunting blinds discovered in Gjesdal, western Norway. Four of the hunting blinds are unique; it was possible to date them directly with radiocarbon dating. The sites exhibit multiple construction phases, with a usage period ranging from the Neolithic to the Mid Iron Age, and we would suggest likely extending into the Late Iron Age and Middle Ages. The datings and multiple phases make it possible to discuss temporal change in the construction of the hunting blinds, which is unprecedented in a Fennoscandian context. Hunting appears to align with social trends, becoming more prominent during periods of settlement decline and increased use of outfield resources for surplus production. While hunting in the lowlands of western Norway, particularly between 0 and 900 metres above sea level, appears to have been smaller in scale compared to mass-scale hunting in Norway’s high-altitude zones and eastern regions, the high population of red deer until about 500 years ago indicates profitable hunting opportunities during specific seasons. The cluster of hunting blinds suggests organized cooperation among neighbours and families, with surplus products potentially sold and exported to local and regional markets. 

The habitat utilization and environmental resilience of Homo heidelbergensis in Europe

The European populations of Homo heidelbergensis may have contributed to the genetic heritage of modern Eurasians. A better understanding of the possible effects of palaeoenvironmental alterations on the evolution of ancient humans can help to understand the origin of developed traits. For this purpose, the spatiotemporal alterations of physical factors were modelled in Europe for the period of 670–190 ka, covering the existence of Homo heidelbergensis in Europe. The factors included the following: paleoclimatic conditions, climatic suitability values of ancient humans, two prey species, and the European beech. Furthermore, the distribution and features of wood used for toolmaking were also investigated. Finally, changes in the relative mortality risk, the percentage of the body covered by clothing, and daily energy expenditure values in the coldest quarter of the year were modelled. The results suggest that H. heidelbergensis inhabited dominantly temperate regions in Europe where prey such as red deer were present. In the northern regions, European beech trees were abundant. When making wood tools, they preferred relatively light but not the strongest woods, which were readily available in the vicinity of the sites. Although hard and heavy woods were also occasionally used, at a European level, significant changes were observed in the relative mortality risk, the percentage of the body covered by clothing, and daily energy expenditure values during the period of 670–190 ka. However, substantial differences between archaeological sites in these values, indicating somewhat ecological variations, were not found during the studied period.

Comparative karyotype analysis of the red brocket deer (M. americana sensu lato and M. rufa) complex: evidence of drastic chromosomal evolution and implications on speciation process.

Chromosomal rearrangements are often associated with playing a role in the speciation process. However, the underlying mechanism that favors the genetic isolation associated with chromosomal changes remains elusive. In this sense, the genus Mazama is recognized by its high level of karyotype diversity among species with similar morphology. A cryptic species complex has been identified within the genus, with the red brocket deer (Mazama americana and Mazama rufa) being the most impressive example. The chromosome variation was clustered in cytotypes with diploid numbers ranging from 42 to 53 and was correlated with geographical location. We conducted an analysis of chromosome evolution of the red brocket deer complex using comparative chromosome painting and Bacterial Artificial Chromosome (BAC) clones among different cytotypes. The aim was to deepen our understanding of the karyotypic relationships within the red brocket, thereby elucidating the significant chromosome variation among closely related species. This underscores the significance of chromosome changes as a key evolutionary process shaping their genomes. The results revealed the presence of three distinct cytogenetic lineages characterized by significant karyotypic divergence, suggesting the existence of efficient post-zygotic barriers. Tandem fusions constitute the main mechanism driving karyotype evolution, following a few centric fusions, inversion X-autosomal fusions. The BAC mapping has improved our comprehension of the karyotypic relationships within the red brocket deer complex, prompting questions regarding the role of these changes in the speciation process. We propose the red brocket as a model group to investigate how chromosomal changes contribute to isolation and explore the implications of these changes in taxonomy and conservation.

Planning for Deer-Hunting Management at the Local and Regional Scales: Reconciling Economic, Social and Ecological Functions

Game species with home ranges exceeding the area of the management units may entail conflicts over hunting rights and cause damage to crops and forest stands in surrounding areas. This is currently the case in the Mendro Mountain Range (Portugal), inhabited by free-ranging red (Cervus elaphus) and fallow deer (Dama dama) populations. This study’s primary goal was to uncover the processes underlying these tensions and identify solutions to overcome them, thus reconciling the economic, social, and ecological functions of hunting. We analyzed data from three different sources of information regarding the surveyed management units: biophysical and anthropical spatial data collected using a GIS; typology, whether fenced, area and game bag results, data provided by a public institute; crop and forest damage locations reported by game managers. Approximately half of the surveyed open management units reported damage. We found no relationship between damage and game bag results, regardless of the typology and habitat quality index. To address this disconnection between the negative and positive values associated with deer locally, we proposed habitat management solutions. It is of chief importance to keep valuable crops apart from deer’s refuge cover, such as bushy areas, to minimize damage in management units where deer hunting is a subsidiary activity. Conversely, in management units where deer hunting is of significant economic importance, the food and refuge cover should be closely interspersed to increase the management unit’s carrying capacity. To improve the efficacy of measures such as this at a regional scale, as in the Mendro Mountain Range, we recommend implementing a so-called Global Management Plan. In Portuguese law, this governance instrument applies to the entire biologic unit where the deer populations occur, thus implying arrangements between the involved stakeholders and multiple other concerned institutions.

Zoogeochemistry of a protected area: Driven by anthropogenic impacts and animal behavior

AbstractAnthropogenic eutrophication of ecosystems is an important driver of biodiversity loss. Even protected areas (PAs) may be impacted by anthropogenic nutrients, for example, from atmospheric deposition or the provision of supplementary feeding. However, the resultant nutrient patterns, and the role of local wildlife in shaping them, remain poorly understood. We investigated anthropogenic influences on the role that red deer (Cervus elaphus) play in the nutrient balance of a PA in Denmark. We used habitat selection modeling and theoretical scenarios where we varied the proportion of energy the deer obtained from supplementary versus natural forage and compared it with the nutrients removed due to hunting. We show that the movement and distribution of the red deer population within the PA are very heterogeneous and likely influenced by the need for shelter. Moreover, depending on their reliance on supplementary feeding, deer can potentially import large amounts of nutrients to the PA, and concentrate them in localized hotspots. However, we also explore the potential for nutrient loss due to hunting activities. Such indirect anthropogenic impacts on nutrient landscapes may counteract restoration and conservation efforts. We therefore recommend incorporating anthropogenic influences on zoogeochemistry and the animal‐mediated connectivity between PAs and anthropogenically dominated landscapes into future management plans.

Red deer responses to drive hunts are related to habitat and landscape characteristics

AbstractHunting triggers behavioral responses in wildlife that may have important consequences for conservation and wildlife management. We studied movement and habitat selection in 51 global positioning system‐collared red deer (Cervus elaphus) in response to 63 large‐scale (x̄ = 18.8 km2) drive hunts conducted in 2015‐2022 on a military training area in southern Germany. The drive hunts were characterized by a low density of beaters and dogs to avoid rapid and long‐distance displacement of red deer. We determined if red deer spatial responses to drive hunts differed in various forest covers, between sexes, and in relation to the individual position prior to the hunt. On the hunting days, red deer increased their hourly displacement rates and shifted their preference for forest versus open habitats from early morning to late morning hours. Individuals initially located within the boundaries of the hunted areas exhibited longer maximum net displacements, had a greater probability of leaving their home ranges, and spent longer times outside of their home ranges than individuals outside the hunted areas. All these parameters were greater in the more forested site than in the less forested site. Males displayed shorter net displacements than females. Red deer moved away (i.e., fled) from their initial positions for variable periods, either only during the hunt or until the next day. The probability of prolonged flight was greater in the more forested site and more likely if the individuals were deeper in the forest in the less forested site. The prolonged flight behavior was desired from the hunters' perspective because red deer displaying this type of response were less likely to leave the hunted area during the hunt. Prolonged flight was associated with greater costs for the red deer, such as greater movement distances after the hunt and longer times outside of home ranges. Wildlife managers should consider that drive hunts may cause prolonged behavioral responses in game that occur both inside and outside the hunted areas. These responses depend on habitat characteristics and may be stronger in more forested landscapes.

Risk response towards roads is consistent across multiple species in a temperate forest ecosystem

Roads can have diverse impacts on wildlife species, and while some species may adapt effectively, others may not. Studying multiple species' responses to the same infrastructure in a given area can help understand this variation and reveal the effects of disturbance on the ecology of wildlife communities. This study investigates the behavioural responses of four species with distinctive ecological and behavioural traits to roads in the protected Bohemian Forest Ecosystem in central Europe: European roe deer Capreolus capreolus, a solitary herbivore; red deer Cervus elaphus a gregarious herbivore; wild boar Sus scrofa, a gregarious omnivore and Eurasian lynx Lynx lynx, a solitary large carnivore. We used GPS data gathered from each species to study movement behaviour and habitat selection in relation to roads using an integrated step selection analysis. For all species and sexes, we predicted increased movement rates in response to roads, selection of vegetation cover near roads and open areas after road crossings, and increased road avoidance during the day. We found remarkably similar behavioural responses towards roads across species. The behavioural adaptations to road exposure, such as increased movement rates and selection for vegetation cover, were analogous to responses to natural predation risk. Roads were more strongly avoided during daytime, when traffic volume was high. Road crossings were more frequent at twilight and at night within open areas offering food resources. Gregarious animals exposed to roads favoured stronger road avoidance over faster movements. Ungulates crossed roads more at twilight, coinciding with commuter traffic during winter. Despite differences in the ecology and behaviour of the four species, our results showed similar adaptations towards a common threat. The continuous expansion of the global transportation network should be accompanied by efforts to understand and minimise the impact of roads on wildlife to assist wildlife management and ensure conservation.

Implementation of PID controller and enhanced red deer algorithm in optimal path planning of substation inspection robots

AbstractIn contemporary power transmission systems, substation monitoring stands as a vital but challenging task. While robotics offers promise in this regard, its potential is still nascent, struggling to replicate human intelligence. This article's core aim was to optimize robot path planning (RPP). Employing the enhanced red deer algorithm (ERDA), we sought to bolster RPP for more efficient substation inspections. The key methods used seem to be modeling, experimentation, comparative analysis, and some elements of data benchmarking to systematically evaluate and validate their proposed technique and models both in simulation and the real world. Research aims to enhance substation inspection effectiveness and bolster the safety of power usage in society. Proposed hybrid approach, combining proportional–integral–derivative (PID) with ERDA (PID–ERDA), underpins an Intelligent Intelligent RPP framework tailored to substation inspections. Examining the PID–ERDA model's performance, it significantly improved path length by 18%–29% and reduced response times by 14%–26% compared with PID or ERDA alone. PID–ERDA consistently achieved optimal solutions in 40–60 trials out of 85, while PID and ERDA managed 20–40 trials with inconsistent optimization. Additionally, it reduced average response times to 17–20 s from 21 to 27 s observed when using PID and ERDA separately. PID–ERDA also demonstrated superior path accuracy, surpassing methods like improved adaptive control algorithm‐feedforward neural network, enhanced unified algorithm‐susceptible‐infected‐removed, and bounded behavior‐particle swarm optimization by 7%–13%. The study affirms that the PID–ERDA model significantly enhances path planning for substation inspections, representing a milestone in RPP for power station inspections within modern power transmission systems. The primary contribution of this research is the significant improvement it brings to RPP for power station inspections, especially in substation monitoring within modern power transmission systems.

The Levels of Cortisol and Selected Biochemical Parameters in Red Deer Harvested during Stalking Hunts.

As a reactive species, the red deer is sensitive to both negative exogenous and endogenous stimuli. An intensive hunting period may have a particularly negative impact on game animals. The aim of this study was to determine the plasma cortisol level and biochemical parameters in 25 wild red deer (Cervus elaphus) harvested during stalking hunts in correlation with the sex and age of the animals. The mean cortisol concentrations in the stags and hinds analyzed in this study were similar (20.2 and 21.5 ng/mL, respectively). Higher HDL cholesterol values were found in the blood of the hinds than in stags (p < 0.05). Similarly, the mean levels of LDL cholesterol, lactate dehydrogenase, and alanine aminotransferase were higher by 21%, 16%, and 42%, respectively, in the blood of the hinds. In contrast, the levels of alkaline phosphatase, bilirubin, and aspartate aminotransferase were higher in the stags (by 30%, 49%, and 36%, respectively). There was a negative correlation of the cortisol concentration with urea and bilirubin and a positive correlation between cortisol and aspartate aminotransferase in the stags (p < 0.05). In turn, a negative correlation was found between the cortisol and urea levels in the hinds (p < 0.05). In summary, the stress caused by stalking hunts and the characteristic behavior of red deer during the mating season had an impact on chosen biochemical parameters. The increased concentration of cortisol resulted in a decrease in the carcass mass, which may lead to the deterioration of the physical condition of animals on hunting grounds.

Late Pleistocene prey mobility in southwestern France and its implications for reconstructing Neandertal ranging behaviors

As hunter-gatherers, Neandertal mobility and corresponding adaptations were influenced by the mobility of their prey; thus, it is critical to track how the movement patterns of each species varied over time at specific sites. Here, prey paleomobility is reconstructed by measuring radiogenic strontium isotope ratios (87Sr/86Sr) in herbivore teeth recovered from two archaeological sites (Pech de l’Azé IV and Roc de Marsal) in the Dordogne Valley (Aquitaine Basin) of southwestern France that span marine isotope stages (MIS) 5-3. These ratios are compared to a published isoscape as well as new environmental samples (soil, Helix shells, rodent teeth, water, and wine). Our results show that reindeer (Rangifer) likely moved within the sedimentary basins of France and did not venture into mountainous regions. Instead, reindeer, bison (Bison) and other prey species were available year-round in the Aquitaine and Paris basins. Red deer (Cervus) and horse (Equus) may have ventured into the mountains. These results suggest that Neandertals could find year-round faunal resources in these basins, at least during MIS 4 and 3.

Diet comparison between sympatric European bison, red deer and fallow deer in a Mediterranean landscape

At the end of 2020, 18 European bison (Bison bonasus) were introduced to El Encinarejo estate, in the south of the Iberian Peninsula. This hunting-oriented estate has 956 hectares of dehesa, Mediterranean forest and scrubland in which about 400 red deer (Cervus elaphus) and 200 fallow deer (Dama dama) coexist with the bison. To know how trophic resources are used by these herbivore species, faecal samples were collected during the year 2021. The microhistological analysis technique was used to determine the percentage of epidermal fragments of the main functional groups (woody plants, graminoids, legumes and forbs) in each sample. The results showed significant differences between animal species and seasons of the year. Globally, woody plants are preferentially consumed in autumn and winter, graminoids in summer and legumes and forbs in spring. The woody component was the most abundant in the diet of the three species, accounting for 81% of the plant fragments present in red deer faeces, 52% in bison and 38% in fallow deer. This is the first study describing the dietary use of Pistacia lentiscus by European bison, the substantial use throughout all seasons indicates it as a very important diet source. In addition, the interspecific comparison showed that the bison significantly consumed more graminoids (21%), whereas legumes were more present in fallow deer diet (32%). Finally, red deer consumed less forbs than the other two species (8%). It is concluded that there is a certain distribution of trophic resources between the three species that may facilitate their coexistence.

Insights from a 31-year study demonstrate an inverse correlation between recreational activities and red deer fecundity, with bodyweight as a mediator.

Human activity is omnipresent in our landscapes. Animals can perceive risk from humans similar to predation risk, which could affect their fitness. We assessed the influence of the relative intensity of recreational activities on the bodyweight and pregnancy rates of red deer (Cervus elaphus) between 1985 and 2015. We hypothesized that stress, as a result of recreational activities, affects the pregnancy rates of red deer directly and indirectly via a reduction in bodyweight. Furthermore, we expected non-motorized recreational activities to have a larger negative effect on both bodyweight and fecundity, compared to motorized recreational activities. The intensity of recreational activities was recorded through visual observations. We obtained pregnancy data from female red deer that were shot during the regular hunting season. Additionally, age and bodyweight were determined through a post-mortem examination. We used two Generalized-Linear-Mixed Models (GLMM) to test the effect of different types of recreation on (1) pregnancy rates and (2) bodyweight of red deer. Recreation had a direct negative correlation with the fecundity of red deer, with bodyweight, as a mediator as expected. Besides, we found a negative effect of non-motorized recreation on fecundity and bodyweight and no significant effect of motorized recreation. Our results support the concept of humans as an important stressor affecting wild animal populations at a population level and plead to regulate recreational activities in protected areas that are sensitive. The fear humans induce in large-bodied herbivores and its consequences for fitness may have strong implications for animal populations.