Implementation Of Strategies Research Articles

Modular chemoenzymatic synthesis of ten fusicoccane diterpenoids.

Fusicoccane diterpenoids display intriguing biological activities, including the ability to act as modulators of 14-3-3 protein-protein interactions. However, their innate structural complexity and diverse oxygenation patterns present enormous synthetic challenges. Here we develop a modular chemoenzymatic approach that combines de novo skeletal construction and late-stage hybrid C-H oxidations to achieve the synthesis of ten complex fusicoccanes in 8-13 steps each. A convergent fragment coupling strategy allowed rapid access to a key tricyclic intermediate, which was subjected to chemical and enzymatic C-H oxidations to modularly prepare five oxidized family members. We also conceived a complementary biomimetic skeletal remodelling strategy to synthetically access five rearranged fusicoccanes with unusual bridgehead double bonds. This work may facilitate future investigation into the biological activities of the fusicoccanes and also inspire the implementation of similar hybrid strategies to provide family-level synthetic solutions to other natural product scaffolds.

Unraveling the Role of Single-Atom Catalysts Immobilized onto Metal–Organic Frameworks in Selective Acetylene Hydrogenation: An Implementation of the Active Site Isolation Strategy

Unraveling the Role of Single-Atom Catalysts Immobilized onto Metal–Organic Frameworks in Selective Acetylene Hydrogenation: An Implementation of the Active Site Isolation Strategy

Digital economy and urban innovation level: A quasi-natural experiment from the strategy of “Digital China”

The digital economy is an essential engine of the innovation-driven development strategy and plays a valuable role in promoting the high-quality development of the regional economy. Based on the panel data of 286 cities in mainland China with the help of the digital economy strategic plans issued by local governments as a quasi-natural experiment, we use the multi-temporal double-difference (DID) method to examine the impact of the digital economy on the urban innovation level. Findings show that the digital economy can significantly improve the urban innovation level. After a series of robustness tests, such as parallel trend test, updating sample and period, and repeated placebo test, the innovation-driving effect generated by the development of the digital economy remains significant. Results of the mechanism analysis indicate that the digital economy enhances the level of innovation through upgrading industrial structures. In addition, we find through the heterogeneity test that the digital economy has a stronger effect on improving the quality of innovation. Meanwhile, the innovation output in the Middle Eastern region is more significantly affected by the “Digital China” strategy than the Western region. Therefore, we should accelerate the implementation of the local digital economy development strategy to realize the high-quality development of the regional economy.

Climate change shocks and crop production: The foodgrain bowl of India as an example

Global warming is causing climate change (CC) characterized by increased frequency of heatwaves, droughts, erratic rains, hailstorms, cloudbursts, floods, landslides etc. The CC has already adversely affected ecosystems. In spite of efforts to mitigate greenhouse gas (GHG) emissions, which lead to warming, the global temperature during 2011–20 was 1.1°C above that during pre-industrial era. The projections are that warming will continue to increase and adverse effects will intensify particularly in developing countries like India. In India a number of studies have recorded wide spatial variability in rainfall, though, many reported a general overall negative trend since mid-20th century. Further, varying pattern of rainfall has been recorded in three agroclimatic regions of Punjab state, the granary of India. Unseasonal rains followed by spiked temperature during winter (rabi) season of 2021–22 reduced wheat (Triticum aestivum L.) yield in Punjab by 651 kg/ha and by 301 kg/ha in Haryana compared to 2020–21. Further, the grain was of lower quality. During rainy (kharif) season of 2022, Southern Rice Black-streaked Dwarf Virus (SRBSDV), appeared for the first time in Punjab and Haryana. Some farmers ploughed the affected fields. Adverse weather during rabi 2022–23 also, reduced the wheat yield (143–150 kg/ha) in these states. At the national level, erratic weather during rabi 2021–22 and kharif 2022 caused losses of about 3 mt of grain of each of wheat and rice (Oryza sativa L.). The projected increased adverse effects due to intensified CC include food insecurity. Thus, there is an emergent need to accelerate implementation of adaptation and mitigation strategies in agriculture. The adaptation options include cultivar improvement, conservation agriculture altering growing seasons, crop diversification and sustainable soil, and water resource management. In the process of adaptive management of crop production, adjusting sowing dates and breeding cultivars having varying duration in consonance with CC has been one of the central aspects. Shifting sowing dates to find appropriate crop cultivation season is a low-cost measure. However, cultivar development is time and resource consuming. Novel biotechnological tools enable fast cultivar development with precision, and facilitate mobilization of genes from wild-weedy relatives, which are rich in genes conferring resistance/tolerance to biotic and biotic stresses, required to combat CC challenge. In view of CC stress on water resources, improving water-use efficiency (WUE) has gained importance. Sensor-based micro-irrigation/fertigation has great potential to enhance water and fertilizer-use efficiency. Similarly, the application of other smart technologies like nanotechnology, sensor-based pesticide application, bio-fertilizers and bio-pesticides, need to be mobilised. In view of agro-ecological diversity in India, right-sized regionspecific technology packages have to be developed implying that crop research will expand exponentially. This needs strengthening of human resources and institutional infrastructure, expanding and linking basic and applied researches, and fortifying inter-disciplinary/inter-institutional collaborations to develop and diffuse technology innovations. Enabling factors include enhanced funding and international cooperation. All-out efforts are needed to have more climate-resilient agriculture.

Power Quality Improvement in Hybrid Power System using STATCOM :

Meeting the escalating electricity demands posed by population growth and industrial expansion presents a significant challenge. Utilizing renewable energy sources like wind, biomass, and hydro co-generation has become imperative to satisfy energy requirements. In this proposed framework, a model featuring a grid-integrated wind energy generation system and nonlinear load is developed using MATLAB/Simulink. Integrating wind energy into the power grid impacts electricity quality, notably manifesting in voltage fluctuations, harmonics, flicker, and poor power factor at the source. The efficacy of a wind turbine and, consequently, electricity quality are assessed. To enhance power quality, the project advocates for the implementation of a STATCOM control strategy in grid-connected wind power systems. A bang-bang controller, based on hysteresis current control, is devised for STATCOM. Integrating STATCOM with the Point of Common Coupling (PCC) mitigates power quality issues. In this envisioned scheme, the FACTS device STATCOM is linked with battery power storage (BESS) at the PCC to alleviate power quality concerns. Incorporating Battery Energy Storage Systems aids in stabilizing the real power source during wind power fluctuations and facilitates the rapid injection of reactive power at the PCC. Keywords: Wind energy, Solar Energy, STATCOM, Electric grid, IGBT, PWM.) etc.

Energy efficiency of ecological buildings in Tunisia: Natural fiber composites and passive strategies impact

Improving the thermal insulation and energy efficiency of building envelopes is a major objective worldwide and has significantly developed in the recent years. This study aims to evaluate the impact of ecological additive and passive strategies on building energy efficiency. An experimental study was carried out to examine the effect of the incorporation of treated Alfa and Posidonia-Oceanica fibers on the thermal properties of cement and gypsum composite samples. The experimental results were then introduced in a numerical study using TRNSYS software to perform a comparison of the energy efficiency and thermal performance of three individual buildings; two ones constructed with our ecological materials and the third one with typical materials is considered as a reference case under the Tunisian climate. The obtained results indicate that the buildings built with Alfa fibers (BAF) and Posidonia-Oceanica fibers (BPOF) are economically effective since they allow a decrease of about 48.20% and 43.48% in heating, 45.71% and 42.77% in cooling, leading to a reduction in CO2 emission of 47.90% and 43.40%, respectively, in comparison with the reference case. The investigation also focuses on the improvement of the ecological building envelope by a storage wall integrated on the south front and shaded by solar movable overhangs during the summer season. The indoor climate results reveal that incorporating passive strategies into the building improves indoor air temperature and preserves a comfortable indoor relative humidity. Heating requirements decrease by 82.82% for BAF and by 79.76% for BPOF. The cooling requirements of the reference building are also reduced by 63.46% for BAF and 60.45% for BPOF by the use of natural night ventilation (4 ACH) and the appropriate shading for Trombe walls and windows. Consequently, the implementation of passive strategies on the ecological buildings led to a net reduction in CO2 emissions by up to 80.55% for BAF, compared to the reference case.

A scoping review of strategies for adolescents' sexual and reproductive health role modelling.

Individuals strive to maintain their sexual and reproductive health (SRH) by being exposed to correct information, having access to contraceptives, and promoting safe sex practices. Adolescent SRH promotion efforts should consider the influence of role models. This review explored the availability and nature of strategies and programmes on adolescents' SRH role modelling and described them using a scoping review. Studies were retrieved from four databases and grey literature through a search of 223 studies. The databases included EBSCO-host, Medline, Sabinet, and Pubmed. Data extraction was guided by a data-extraction tool adapted from the JBI Manual for Evidence Synthesis. The characteristics of the selected studies were recorded in a Microsoft spreadsheet. Eleven studies published between 2014 and 2022 were included for the final review and analysed using thematic analysis. Selected articles focused on adolescents' SRH; however, only two studies focused particularly on role modelling. Nonetheless, some aspects of the findings and recommendations presented could be extrapolated to adolescents' SRH role modelling. This includes adolescent-parent communication on SRH, community engagement, mentoring, positive role modelling, and information sharing through media campaigns. There is a lack of literature on SRH role modelling because most studies did not focus on role modelling as an aspect of SRH. Therefore, research needs to be conducted on strategies and programmes focusing on SRH modelling.Contribution:The findings of this scoping review may encourage the development and implementation of strategies and programmes targeting adolescents' SRH throughout diverse communities to promote adolescent SRH.

Toxicity burden patterns of MET-selective tyrosine kinase inhibitors: evidence from real-world pharmacovigilance.

MET exon 14 skipping alterations and MET amplifications are recognized as oncogenic and targetable genetic changes in cancer patients. The treatment of MET-selective tyrosine kinase inhibitors (TKIs) in this specific population has shown encouraging therapeutic results. However, a comprehensive understanding of the potential toxicities linked to these agents is still lacking.The present pharmacovigilance analysis was carried out using the FDA Adverse Event Reporting System database to assess notable adverse events associated with MET-selective TKIs.Gastrointestinal disorders, respiratory toxicity, hepatotoxicity, and disturbances in metabolism and nutrition demonstrated a substantial prevalence and significance among the adverse event (AE) categories. Particularly notable were the occurrences of peripheral oedema, nausea, dysphagia, fatigue, and dyspnoea, which emerged as the foremost five reported AEs. The majority of these AEs were observed within the initial months of initiating treatment with MET-selective TKIs and persistently thereafter. Notably, our investigation unveiled a significant correlation between the usage of capmatinib and the incidence of hearing loss and difficulty in swallowing.Diligent monitoring and the implementation of supportive care strategies are essential in managing the toxicities associated with MET-selective TKIs, particularly those related to gastrointestinal disorders, respiratory toxicity, hepatotoxicity, and ototoxicity.

Abstract PO1-12-03: Quality of Life in Patients Diagnosed and Treated for Breast Cancer in Uruguay

Abstract Introduction: Breast cancer (BC) and its treatment can impair patient quality of life (QOL), and those undergoing more aggressive treatments may be more severely impacted. Objective: Assess the level of perception of the QOL of patients treated for BC at the Hospital de Clínicas and the Hospital Departamental de Soriano. Materials and Methods: A questionnaire for cancer patients (EORTC, QLQ-C30) and one specific for BC (EORTC QLQ-BR23) were used. Results: A total of 158 patients who had completed chemotherapy treatment at least one year prior to the evaluation were enrolled. The average age was 61 years old. QLQ-C30 questionnaire: the global QOL score (GQOL) was high: 70.9. Patients undergoing conservative surgery (CS) had better scores in physical and emotional functioning (p < 0.005) and presented less frequently with: pain, constipation and financial difficulties (p < 0.005). Those undergoing sentinel lymph node biopsy (SLNB) had higher scores for GQOL and for physical, role and social functioning scales (p < 0.001) and had less fatigue, pain, insomnia and financial difficulties (p < 0.005). Questionnaire QLQ-BR23: sexual functioning and sexual enjoyment scales were relatively low. Patients undergoing CS had better scores on the functional scales: body image and future outlook; and fewer breast symptoms (p < 0.005). Those undergoing SLNB also had better scores on the functional scales for body image and future outlook future and presented less frequently with symptoms (p < 0.005). Conclusion: Patients had high values on the GQOL scale, those undergoing CS and SLNB had better scores on most functional and problem/symptom scales. The implementation of intervention strategies aimed at improving the quality of life and the physical and emotional care of patients is recommended. Citation Format: Natalia Camejo, Dahiana Amarillo, Cecilia Castillo, Maria Guerrina, Florencia Savio, Mariana Carrasco, Noelia Strazzarino, Ana laura Hernandez, Guadalupe Herrera, Gabriel Krygier. Quality of Life in Patients Diagnosed and Treated for Breast Cancer in Uruguay [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO1-12-03.

Abstract PO5-12-03: Cardiovascular Toxicities in Breast Cancer Survivors

Abstract Background: Chemotherapy, despite its effectiveness, presents drawbacks in breast cancer patients due to its side effects. Fatigue, hair loss, nausea, and anemia are among the many symptoms associated with chemotherapy. Moreover, chemotherapy can lead to cardiovascular diseases, including cardiac arrhythmias, hypertension, heart failure, heart attacks, and cardiomyopathy, particularly with the use of anthracyclines and HER-2-targeted agents. Risk factors such as weight, age, race, comorbidities (e.g., obesity, diabetes, hyperlipidemia), and radiation exposure further increase these risks. Despite these potential complications, guidelines are just emerging aiming to prevent these cardiovascular complications in breast cancer patients. Untreated cardiovascular abnormalities can have long-term effects on breast cancer survivors. Our aim is to analyze the relationships between breast cancer treatment and cardiovascular abnormalities, examining associations with various factors, including race, BMI, and age in a majority Hispanic cohort of breast cancer survivors. Methods: retrospective, single institution study data from a total of 53 patients treated with chemotherapy with either anthracyclines and/or HER 2 directed therapy with curative intent between 2018-2020. Our analysis included basic demographic information, menopausal status, breast cancer type, treatment types, use of cardio-protective medications, and cardiovascular effects (both pre-existing and developed during treatment). Results: Our sample of 50 women primarily comprised Hispanic individuals (73%), most of whom were postmenopausal (86%), with an average age of 63 years (range 35-86). Among the breast cancer types identified, 35 were hormone-positive, 8 were HER-2 positive, and 10 were triple negative. 72% of the patients received adjuvant hormonal therapy. 18% of the population received anthracycline-based treatment, 8% received Her 2 directed therapy and 2 % received Immune check point inhibitors. Radiation therapy was administered to 50% of the patients. The most prevalent cardiovascular disease observed was hypertension, affecting 62% of patients, followed by type 2 diabetes (38%), hyperlipidemia (34%), stroke (8%), and heart attack (6%). The predominant cardioprotective medications used were ACE inhibitors/ARBs (48%), followed by statins (46%), beta-blockers (20%), aspirin (16%), and GLP-1 agonists (14%). Conclusion: Our analysis revealed a high prevalence of cardiovascular conditions among breast cancer survivors treated with chemotherapy in a majority Hispanic cohort of patients. This population has been linked to higher risk of developing comorbidities such as diabetes, obesity and hypertension. Further analysis evaluating risk factors and implementation of treatment strategies to minimize adverse effects is needed in this population. Citation Format: Victoria Ayodele, Emily Sherry, Marcela Mazo-Canola. Cardiovascular Toxicities in Breast Cancer Survivors [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO5-12-03.

Abstract PO2-25-03: Deciphering the immune molecular signature associated with pembrolizumab efficacy in the neoadjuvant setting of Triple-Negative Breast Cancer patients: a single-centre experience

Abstract Triple-negative breast cancer (TNBC) accounts for 15-20% of all breast cancers and is characterized by a high rate of recurrence, despite the improvement of the therapeutic approaches in recent years. Neoadjuvant chemotherapy (NACT) is the standard treatment for early-stage TNBC. Achieving a pathological complete response (pCR) is considered an essential prognostic factor for long-term outcomes. However, the percentage of pCR patients after NACT is limited to 30-40%, thus requiring the implementation of novel therapeutic strategies. Recently, by applying a morphology-guided spatial transcriptomic approach on Tru-Cut biopsies, we showed that intratumoral infiltrating lymphocytes (iTILs) and stromal Natural Killer (NK) cells are major determinants of chemotherapy efficacy in TNBC. These data highlight a complex three-dimensional organization of the tumor landscape and underline how spatiality and molecular activation of immune cells are essential to enhance the efficacy of neoadjuvant NACT in TNBC. The fact that immune cells are important players in this setting is corroborated by the clinical success shown by the KEYNOTE-522 study. This trial paved the way for the use of immunotherapy in neoadjuvant settings, obtaining a significant improvement in the patient’s overall response compared to standard NACT. Still, the implementation of chemotherapy with immunotherapy in the TNBC neoadjuvant treatment implicates several compelling questions, including how to further increase the percentage of patients with a complete response, overcoming resistance mechanisms, and how to improve the personalization of treatment, evaluating which patients can benefit from a therapy de-scalation or another combined therapeutic strategy. In this prospective cohort study, we decided to define the relevance of different immune populations in affecting pembrolizumab efficacy in the neoadjuvant treatment of TNBC patients. We treated 40 patients with an early-TNBC diagnosis with the KEYNOTE-522 regimen that consists of four cycles of an intravenous infusion of pembrolizumab plus paclitaxel and carboplatin, followed by four cycles of pembrolizumab plus epirubicin and cyclophosphamide. Only patients with evident side effects to immunotherapy administration were a priori excluded. Currently, 18 patients ended the neoadjuvant schedule and underwent surgical excision. Our data indicate that 15 patients displayed a pCR at the end of the therapy, whereas 3 patients displayed only partial response. To further clarify the immune dynamics that lead to a complete response to pembrolizumab in these patients, we collected peripheral blood before and after neoadjuvant treatment to isolate peripheral blood mononucleated cells (PBMCs). We applied a single-cell transcriptome analysis to shape any variations in circulating immune cells occurring during neoadjuvant treatment and associated with patient response. In addition, we collected residual tumors at surgery from partial responders and derived three-dimensional organoids that recapitulate the characteristics of the original patient tumor. These ex vivo organoid-based platforms are applied to clarify the molecular mechanisms driving neoadjuvant resistance. To this aim, we are performing co-cultures with autologous immune cells to identify novel combined therapeutic strategies to rescue pembrolizumab efficacy in these models. Overall, our study is defining the immune-related molecular mechanisms driving pembrolizumab and chemotherapy response in the neoadjuvant treatment of TNBC patients. Our findings can improve our understanding of which immune populations are determinant players in anti-TNBC immune response and can be proposed as novel biomarkers of pembrolizumab efficacy during the neoadjuvant treatment. Citation Format: Elisa Gasparini, Gloria Manzotti, Moira Ragazzi, Benedetta Donati, Federica Torricelli, Elisa Salviato, Alessandra Bisagni, Eleonora Zanetti, Alessandra Bologna, Saverio Coiro, Rita Vacondio, Guglielmo Ferrari, Giancarlo Bisagni, Carmine Pinto, Alessia Ciarrocchi, Francesca Reggiani. Deciphering the immune molecular signature associated with pembrolizumab efficacy in the neoadjuvant setting of Triple-Negative Breast Cancer patients: a single-centre experience [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO2-25-03.

Abstract PO5-15-10: Plasticity marker FOXC1 expression accurately predicts efficacy of Adjuvant Tamoxifen + Chemotherapy in reducing all-cause mortality in ER+LN- Breast Cancer: Validation in the SCAN-B Prospective Study (NCT02306096)

Abstract INTRODUCTION: Despite the development and validation of multimarker gene panels like OncotypeDx®, Mammaprint® and EndoPredict®, an affordable and globally accessible biomarker approach that can predict increased risk of all-cause mortality in patients diagnosed with ER+LN- breast cancer, as well as the efficacy of adjuvant endocrine + chemotherapy in mitigating such elevated risk, continues to be an unmet medical need. Lack of such a pragmatic solution continues to be the cause of preventable recurrent/metastatic disease in patients diagnosed with ER+LN- breast cancer in resource-challenged settings around the world. We hypothesized that a predictive biomarker strategy that measures expression of the plasticity marker FOXC1, in combination with clinical parameters like tumor size (TS) and tumor grade (TG), may offer equivalent results to that of the above multimarker gene panels at a significantly lower economic cost without compromising accuracy of prediction results. METHODS: Pre-treatment tumor RNA data obtained from a training cohort (compendium of gene expression microarray datasets, n=2857) and a single large validation cohort (SCAN-B Prospective Multicenter Observational Study, n=3520) for patients diagnosed with ER+LN- breast cancer were analyzed for FOXC1 expression, tumor size (TS) and tumor grade (TG) and correlated with Recurrence-Free Survival (RFS) and Overall Survival (OS). Optimized biomarker cutoff values based on model area-under-curve were leave-one-out cross validated and Risk-of-Recurrence (ROR) prediction algorithm derived utilizing the (compendium) training dataset. The unmodified strategy was then validated in the independent (SCAN-B) validation dataset. RESULTS: A predetermined High ROR score calculated using FOXC1 expression, TS and TG (trained using the compendium dataset) predicted efficacy of adjuvant endocrine + chemotherapy over that of adjuvant endocrine therapy alone in ER+LN- patients in terms of statistically significant reduction in all-cause mortality at 8-years post-diagnosis in the SCAN-B validation dataset (4.66% vs 24.06%, n=326, OR=6.48, 95%CI [2.97-14.11], p< 0.0001). OncotypeDx® (5.7% vs 14.4%, n=762, OR=2.79, 95%CI [1.37-5.67], p=0.002), Mammaprint® (5.1% vs 18.4%, n=665, OR= 95%CI [2.04-8.43], p< 0.0001) and Endopredict® (4.8% vs 15.2%, n=923, OR=3.58 95%CI [1.78-7.21], p=0.0002) were also statistically significant predictors of the same. CONCLUSION: Pre-treatment tumor FOXC1 mRNA or protein expression (assessed using qRT-PCR or routine immunohistochemistry (IHC), respectively, when combined with TS and TG presents a unique and economical alternative solution to multimarker gene panel tests like OncotypeDx®, Mammaprint® or Endopredict®, for guiding therapy of patients diagnosed with ER+LN- breast cancer in resource challenged settings. Such an approach to identify elevated risk of recurrence in patients diagnosed with ER+LN- breast cancer and prevent the same by guiding adjuvant endocrine + chemotherapy decisions, could help to extend recurrence-free and overall survival. Such an approach merits testing in real world ER+LN- patient cohorts in resource-challenged settings to help support implementation of this FOXC1-driven predictive biomarker strategy in the clinic. Citation Format: Partha Ray, Tania Ray, Clive Taylor, Robert Hussa. Plasticity marker FOXC1 expression accurately predicts efficacy of Adjuvant Tamoxifen + Chemotherapy in reducing all-cause mortality in ER+LN- Breast Cancer: Validation in the SCAN-B Prospective Study (NCT02306096) [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO5-15-10.

Systematic review of COVID-19 vaccine acceptance and hesitancy: global perspectives and implications.

The acceptance rate towards COVID-19 vaccination among the global population could be higher due to the need for more education or awareness of prevention strategies to control the COVID-19 outbreak. Thus, there is a dire need to reduce the population’s reluctance towards COVID-19 vaccination across the globe, which will ultimately lead to the implementation of prevention and control strategies against the COVID-19 virus and improving health outcomes across the globe. The following study intends to conduct a systematic review of the literature to evaluate the relationship between COVID-19 vaccination and the current trends related to COVID-19 vaccination faced by the global population. The methodology employed in this study primarily focused on assessing secondary data by conducting a systematic literature review. Internet and electronic databases were used to select research articles to obtain secondary sources. Six articles were selected from ScienceDirect and Google Scholar for a systematic literature review. The keywords used for the selection of articles were “COVID-19,” “Global trends,” Reluctance, “and “vaccination.” The selected articles that were most suitable for the topic consisted of global trends related to COVID-19 vaccination. Six articles relevant to the topic were selected and reviewed. The selected articles showed data collection results from different research journals. The findings of the six research articles depicted common themes, such as each vaccine having a different level of access attitude towards the regional existence, which primarily depends on the variability to determine the vaccine’s effectiveness. Further studies are required to evaluate the factors and strategies that help healthcare organizations and governments reduce the reluctance to use the COVID-19 vaccine among populations worldwide.

Pteridophytes and lycophytes from Osomba Hills, Cross River National Park, Nigeria

Pteridophytes and lycophytes from Osomba Hills, a biodiversity hotspot in Nigeria, were collected using a transect method. Floristic sampling was done in six 100 m transects and elevation range of 170 m – 405 m above sea level where each transect consisted of five 10 m × 10 m quadrats spaced 10 m apart. Fieldwork was performed during the dry (February) and wet seasons (July) in 2022. F ifty species from 22 genera in the nine families Adiantaceae, Aspleniaceae, Cyatheaceae, Dennstaedtiaceae, Hymenophylaceae, Polypodiaceae, Pteridaceae, Schizaeaceae and Selaginellaceae were recorded. The lycophytes were represented by only one family, the Selaginellaceae, while the remaining eight families are pteridophytes. The family with the highest number of species was Polypodiaceae (21), while Cyatheaceae and Schizaeaceae recorded only 1 species each. Nine species were collected only in the dry season while 20 species were collected during the wet season. The remaining 21 species were identified in both dry and wet seasons. T he tree fern Alsophila camerooniana (Hook.) R. M. Tryon and the three species of Hymenophyllaceae were limited in range of occurrence. The diversity of these ferns was significant (P<0.05) within Osomba Hill. This variance was typified along latitudinal and temporal scales. The conservation status of 47 species were categorized as ‘Not Evaluated’. A comprehensive evaluation of the conservation status of these plants is recommended to enable the implementation of an appropriate conservation strategy by the management of the park.

Assessment of the acceptance and implementation of child survival strategies among mothers in some selected communities in south-south Nigeria

Objective: The aim of this study was to assess the acceptance and implementation of child survival strategies through knowledge and practices among mothers in some selected communities in south-south Nigeria. Method: A descriptive survey method was used for the study and Rivers state which is in South-South Nigeria was randomly selected for the pilot study. The study was conducted between September 2021 and August 2023 and a sample size of Six Hundred respondents were randomly selected from the three senatorial districts of Rivers State. The instrument used for data collection was researchers’ constructed questionnaire with 17 items grouped into A and B. Section A contained information on demographic data of the respondents while section B focused on the Knowledge, practices and factors that influenced the acceptance and implementation of child survival strategies among mothers in some selected Local Government Areas in Rivers state. Data was analysed using descriptive statistics of percentages in frequency and tables. Results: The results of the findings showed a high level of knowledge (100%) and its components like skilled child birth attendance (92%), exclusive breastfeeding (83%), immunization (96%) and family planning (78.5%). Also majority of the mothers actually practiced the components of child survival strategies in this study, except family planning services (16%) despite being in the peak of their reproductive years and already having between four to six children. Other factors such as the attitude of the health workers and educational background of mothers were also identified as barriers to the practice of child survival strategies. Conclusion: It was therefore recommended amongst others that government at all levels should embark on aggressive health enlightenment campaign to further sustain these practices and also improve the acceptance and implementation of the practices of other child survival strategies yet to be accepted by mothers.

Strategies and Interventions to Support Quality Outcomes in the Home Care Setting: A Longitudinal Multilevel Study.

The relationship between the practice environment, empowerment, and outcomes such as quality of care, job satisfaction, and intent to stay has been extensively studied in healthcare settings, including hospitals and long-term care facilities. Research consistently demonstrates that a positive practice environment, characterized by supportive leadership, adequate resources, and opportunities for professional growth, are associated with better quality of care, increased job satisfaction, and higher intent to stay among healthcare professionals. Limited knowledge exists regarding the specific relationship between the practice environment, empowerment, and these outcomes within home care organizations. This study assesses the impact of strategies on nurse practice environment, social capital, decision latitude, workload, care quality, job satisfaction, and retention in a Belgian home healthcare organization. A longitudinal survey was conducted in a home healthcare organization, with data collected at 3 time points: baseline (T1) (2015), T2 (2018), and T3 (2021). In T3, respondents reported significantly higher scores for departmental and general management compared with T1. The interventions led to significant improvements in social capital and decision latitude. Self-reported quality of care at the department level showed a significant increase, whereas no significant change was observed for quality of care during the last round. The implementation of strategies and interventions as part of a broader healthcare transformation process had a positive impact. Improvements were observed in nurse-reported quality of care, job satisfaction, and intent to stay in nursing. These findings emphasize the effectiveness of the implemented measures in enhancing nursing practice and creating a positive work environment. Continuous efforts to implement and evaluate such strategies are essential for enhancing the satisfaction and retention of nursing teams within healthcare organizations.

Phytoparasitic and free-living nematodes associated with the cultivation of Passiflora ligularis Juss. in the Sandia Valley, Puno Region, Peru

The identification of phytoparasitic nematodes, which is essential for the implementation of management strategies, is required for further study. The objective of this work was to identify, at the genus level, the phytoparasites and free-living nematodes associated with the cultivation of sweet granadilla (Passiflora ligularis Juss.) in the valley of Sandia, region Puno, Peru. For the study, 165 soil samples were evaluated, coming from eight communities from the district of Sandia, during the agricultural season 2018–2019. The samples were processed by the method of centrifugal fluctuation in sucrose solution, later identified on the basis of morphological characteristics. The results showed the presence of seven genera of phytoparasitic nematodes, Meloidogyne, Pratylenchus, Helicotylenchus, Mesocriconema, Xiphinema, Tylenchus, and Hemicycliophora, and two genera of free-living nematodes, Aphelenchus and Dorylaimus. The most harmful genera were Meloidogyne and Pratylenchus because they cause damage to other crops. In the nematode community, there was variability in the density and frequency of phytoparasitic and free-living nematodes in the evaluated localities.

Implementation of Training Strategies, Financial Management, Human Resource Management, Business Management, Layer Chicken

This article explores the implementation of financial management, human resource management (HRM), and business management training strategies in the context of layer chicken farming in Indonesia. Using a case study method, this research investigates best practices and challenges faced in implementing these strategies. The findings indicate that training in financial management, HRM, and business management significantly enhances operational efficiency and profitability of layer chicken farming businesses. However, the implementation of these strategies also encounters several obstacles such as limited resources, resistance to change, and a lack of understanding of the importance of effective management. This article provides valuable insights for layer chicken farming business owners and other stakeholders to strengthen management practices in the poultry industry. Further steps are also suggested to address the challenges encountered in implementing these strategies.

A Review of Factors Influencing Strategy Implementation in the Public Sector

This article presents a review of strategy implementation in the public sector. The findings show that several researchers have highlighted the need for a more comprehensive investigation of strategy implementation in the public sector, whereby specific public sector concepts should be considered. A framework for internal and external factors of strategy implementation is developed based on the available evidence. The framework includes recommendations for the the gaps addressed beforehand: 1. To link strategy implementation based on public sector concept with internal factors such as centralised, formalised and complexity structure, transformational and transactional leadership, formal and informal communication, and appropriate organisational culture, and 2. To consider the impact of external factors such as political and governmental regulation, resources from the budgeting system and technology infrastructure on the internal factors as moderators.

Targeting strategies of antenatal balanced energy and protein supplementation in Addis Ababa, Ethiopia: study protocol for a randomized effectiveness study

BackgroundAntenatal balanced energy and protein (BEP) supplements have well-documented benefits for pregnancy outcomes. However, considerable practical gaps remain in the effective and cost-effective delivery of antenatal BEP supplements at scale in low- and middle-income countries.MethodsA randomized effectiveness study will be conducted in two sub-cities of Addis Ababa, Ethiopia, to evaluate the effectiveness, cost-effectiveness, and implementation of different targeting strategies of antenatal BEP supplements. Pregnant women aged 18 to 49, with a gestational age of 24 weeks or less, and attending antenatal visits in one of the nine study health facilities are eligible for enrollment. In six of the health facilities, participants will be randomized to one of three study arms: control (Arm 1), targeted BEP provision based on baseline nutritional status (Arm 2), and targeted BEP supplementation based on baseline nutritional status and monthly gestational weight gain (GWG) monitoring (Arm 3). In the remaining three facilities, participants will be assigned to universal BEP provision (Arm 4). Participants in Arms 2 and 3 will receive BEP supplements if they have undernutrition at enrollment, as defined by a baseline body mass index less than 18.5 kg/m2 or mid-upper arm circumference less than 23 cm. In Arm 3, in addition to targeting based on baseline undernutrition, regular weight measurements will be used to identify insufficient GWG and inform the initiation of additional BEP supplements. Participants in Arm 4 will receive BEP supplements until the end of pregnancy, regardless of baseline nutritional status or GWG. All participants will receive standard antenatal care, including iron and folic acid supplementation. A total of 5400 pregnant women will be enrolled, with 1350 participants in each arm. Participants will be followed up monthly during their visits to the antenatal facilities until delivery. Maternal and infant health status will be evaluated within 72 h after delivery and at 6 weeks postpartum. The effectiveness and cost-effectiveness of the different BEP targeting strategies in preventing adverse pregnancy outcomes will be compared across arms. Qualitative data will be analyzed to assess the feasibility, acceptability, and implementation of different supplementation strategies.DiscussionThis study will inform global recommendations and operational guidelines for the effective and cost-effective delivery of antenatal BEP supplements. The targeted approaches have the potential for broader scale-up in Ethiopia and other low-resource settings with a high burden of undernutrition among pregnant women.Trial registrationClinicalTrials.gov registration number: NCT06125860. Registered November 9, 2023.