Gold Nanoparticles In Solution Research Articles

A novel dye-sensitised solar cell based portable sensor for chemical detection

In recent years, chemical sensors have shown the potential to provide rapid, accurate, and on-site quantitative detection of analytes, but current methods often require specialised equipment. In this study, a novel dye-sensitised solar cell (DSSC) based sensor is reported for convenient detection of tert-butylhydroquinone (TBHQ) in drinking water. The sensor consists of a gold nanoparticle (AuNP) solution based unit that responds to the concentration of TBHQ and produces an electrical signal change via an integrated DSSC based transducer component. Through analysing the current signal generated by the DSSC sensor, the quantity of TBHQ in an unknown sample can be achieved. Finite difference time domain (FDTD) simulation has been used to explain the relationship between the particle morphologies and optical properties of AuNPs. This work offers a novel strategy of developing portable sensors without needing any scientific instrument.

Effect of Aspect Ratio of a Gold-Nanorod-Modified Screen-Printed Carbon Electrode for Carbaryl Detection in Three Different Samples of Vegetables.

In this study, three different sizes of gold nanorods (AuNRs) were synthesized using the seed-growth method by adding various volumes of AgNO3 as 400, 600, and 800 μL into the growth solution of gold nanoparticles. Three different sizes of AuNRs were then characterized using UV-vis spectroscopy, high-resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED) patterns, and atomic force microscopy (AFM) to investigate the surface morphology, topography, and aspect ratios of each synthesized AuNR. The aspect ratios from the histogram of size distributions of three AuNRs as 2.21, 2.53, and 2.85 can be calculated corresponding to the addition of AgNO3 volumes of 400, 600, and 800 μL. Moreover, each AuNR in three different aspect ratios was drop-cast onto the surface of a commercial screen-printed carbon electrode (SPCE) to obtain three different SPCE-modified AuNRs (SPCE-A400, SPCE-A600, and SPCE-A800, respectively). All SPCE-modified AuNRs were then evaluated for their electrochemical behavior using cyclic voltammetry and electrochemical impedance spectroscopy (EIS) techniques and the highest electrochemical performance was shown as the order of magnitude of SPCE-A400 > SPCE-A600/SPCE-A800. The reason for the highest electrocatalytic activity of SPCE-A400 might be due to the smallest particle size and uniform distribution of AuNRs ∼ 2.2, which enhanced the charge transfer, thus providing the highest electroactive surface area (0.6685 cm2) compared to other electrodes. These results also confirm that the sensing mechanism for all SPCE-modified AuNRs is controlled by diffusion phenomena. In addition, the optimum pH was obtained as 4 for carbaryl detection for all SPCE-modified AuNRs with the highest current shown by SPCE-A400. Furthermore, SPCE-A400 has the highest fundamental parameters (surface coverage, catalytic rate constant, electron transfer rate constant, and adsorption capacity) for carbaryl detection, which were investigated using cyclic voltammetry and chronoamperometric techniques. The electroanalytical performances of all SPCE-modified AuNRs for carbaryl detection were also investigated with SPCE-A400 displaying the best performance among other electrodes in terms of its linearity (0.2-100 μM), limit of detection (LOD) ∼ 0.07 μM, and limit of quantification (LOQ) ∼ 0.2 μM. All SPCE-modified AuNRs were also subsequently evaluated for their stability, reproducibility, and selectivity in the presence of interfering species such as NaNO2, NH4NO3, Zn(CH3CO2)2, FeSO4, diazinon, and glucose and show reliable results as depicted from %RSD values less than 3%. At last, all SPCE-modified AuNRs have been employed for carbaryl detection using a standard addition technique in three different samples of vegetables (cabbage, cucumber, and Chinese cabbage) with its results (%recovery ≈ 100%) within the acceptable analytical range. In conclusion, this work demonstrates the great potential of a disposable device based on an AuNR-modified SPCE for rapid detection and high sensitivity in monitoring the concentration of carbaryl as a residual pesticide in vegetable samples.

High-Precision Optical Excited Heaters Based on Au Nanoparticles and Water-Soluble Porphyrin

Gold nanoparticles are widely used as local heaters under optical excitation. Hybrid molecular-plasmon nanostructures based on gold nanoparticles and water-soluble porphyrin have been developed. A colloidal solution of gold nanoparticles was obtained by laser ablation of metallic gold in water, ensuring its highest chemical purity. The hybrid nanostructures formation was performed due to the Coulomb interaction of cationic porphyrin and gold nanoparticles. The revealed functional properties of hybrid nanostructures make them promising for controllable nano-heater applications (for example, photothermal therapy). Gold nanoparticles act as heaters, whereas porphyrin serves as a fluorescent thermometer with a single optical excitation.

Multivariate Analysis of Protein-Nanoparticle Binding Data Reveals a Selective Effect of Nanoparticle Material on the Formation of Soft Corona.

When nanoparticles are introduced into the bloodstream, plasma proteins accumulate at their surface, forming a protein corona. This corona affects the properties of intravenously administered nanomedicines. The firmly bound layer of plasma proteins in direct contact with the nanomaterial is called the "hard corona". There is also a "soft corona" of loosely associated proteins. While the hard corona has been extensively studied, the soft corona is less understood due to its inaccessibility to analytical techniques. Our study used dynamic light scattering to determine the dissociation constant and thickness of the protein corona formed in solutions of silica or gold nanoparticles mixed with serum albumin, transferrin or prothrombin. Multivariate analysis showed that the nanoparticle material had a greater impact on binding properties than the protein type. Serum albumin had a distinct binding pattern compared to the other proteins tested. This pilot study provides a blueprint for future investigations into the complexity of the soft protein corona, which is key to developing nanomedicines.

Experimental and Theoretical Study of Biosurfactants Functionalized Gold Nanoparticles for Mixture Detection and Chiral Recognition of Tryptophan by UV-VIS Spectroscopy

Background: Tryptophan (Trp) is an essential amino acid and plays important roles in biological processes. The detection of Trp is very important for its biological and chemical study. Moreover, Trp is a chiral compound; due to its importance in biological processes, researchers have been long committed to the chiral recognition and sensing of Trp enantiomers. Methods: Two biosurfactants, sodium cholate and sodium deoxycholate, were used for the preparation of functionalized gold nanoparticles (AuNPs) which were characterized by transmission electron microscope and potentiometer. UV-Vis spectra of functionalized gold nanoparticle solutions with different concentrations of Trp, tyrosine, phenylalanine, D-Trp, and L-Trp were analyzed. Then, the discrimination mechanism was further investigated, and the promotion mechanism of biosurfactants was studied by density functional theory (DFT). Results: Trp could induce the aggregation of unmodified AuNPs in 2 h, while phenylalanine and tyrosine could not. Adding biosurfactants promoted the aggregation process, and D- Trp rather than LTrp was found to be responsible for the aggregation. Therefore, there were interaction differences not only between Trp, phenylalanine, and tyrosine but also between Trp enantiomers. Conclusions: UV-vis spectroscopy could be applied for the direct detection of Trp in mixtures as well as the chiral recognition of Trp enantiomers. DFT calculations proved that the interactions of D-Trp with biosurfactants were the strongest, which contributes to the promotion of aggregation.

Theoretical investigation on the effect of volume fraction in the optical trapping of gold nanoparticles

Stable optical trapping of gold nanoparticles is essential and desirable because of its wide applications in nanotechnology. While several factors have been proposed to affect optical trapping stability, the sample’s volume fraction during optical trapping has often been neglected. To address this, by utilizing the effective medium theory, we analyze the stability of optical trapping of a gold nanoparticle in human serum albumin solutions, HIV-1,virus solutions, and gold nanoparticle solutions in this article. Our comparative analysis of the optical force and potential on a single gold nanoparticle in solutions of varying volume fractions reveals that both parameters decrease with increasing volume fraction. This finding can aid in more effective control of gold nanoparticles in various applications.

Headspace single-drop microextraction combined with nanodrop spectrophotometry for ultra-trace detection of ethanethiol using a suspended drop of AuNPs as a plasmonic sensor

It is challenging to achieve ethanethiol extraction, enrichment in a single-drop microextraction system, and an assay with good sensitivity and selectivity for analyzing food analytes in complex matrices. In this study, we combined headspace single-drop microextraction (HS-SDME) with nanodrop array spectrophotometry applied for ultra-trace determination of ethanethiol. A suspended drop of gold nanoparticles (AuNPs) solution was utilized for sensing ethanethiol as an acceptor phase. The thiol portion of ethanethiol molecules binds to the negatively charged AuNPs. Thus, the aim of target enrichment was attained, while matrix effects interference was also minimized. The approach can be used for the direct detection of ethanethiol in food without any sample pretreatment. The obtained range of linearity was 1–1000 µM and 100–1000 nM. The detection limits were 66.62 nM, comparable with those of previously reported methods. The method successfully determined ethanethiol in food (beverage, non-alcoholic beer, and cheese).

Hydrophobicity and FTIR absorbance spectroscopy of Polydimethylsiloxane (PDMS) films treated with aqueous gold nanoparticles (AuNP) solution

Polydimethylsiloxane (PDMS) thin films were treated with aqueous solutions of gold nanoparticles. Samples treated for 24, 48, and 72 h, including untreated PDMS film, were subjected to hydrophobicity test and FTIR absorbance spectroscopy. Hydrophobicity test involved measuring the contact angle of water droplets placed on top of the sample films. Contact angles were measured using Image J software. Treated samples showed significant hydrophobicity changes shown by the increasing value of measured contact angle from 71.3° for the untreated sample to 104.0° for the sample treated for 72 h. FTIR absorbance spectra of the treated sample showed peaks at 3374 cm−1, 3295 cm−1, 2096 cm−1 and 2031 cm−1 with the peak intensity values increasing with corresponding samples of increasing hours of treatment. With the presence of gold nanoparticles on the PDMS film confirmed, the gold nanoparticles may now be functionalized for biosensing and biotesting applications of liquid samples.

Wavelength-dependence of the photothermal efficiency of gold nanoparticles in solution by Z-scan photothermal lens spectroscopy

Using continuous-wave (cw) single-beam Z-scan technique, the absorption and scattering efficiencies of spherical gold nanoparticles (NPs) of varying diameter are determined experimentally through the thermal lens (TL) effect for three different wavelengths in the blue, the green and the red. The main characteristics of the measurement method are detailed, extending the usual experimental procedure for weak absorption with Gaussian beams to higher absorption with top-hat beams. To confirm the validity of our approach, the experimental TL data were compared to the theoretical values from Mie theory and found to be in good agreement within the experimental errors. As expected, the photothermal efficiency of spherical gold NPs increases with lower diameters and towards shorter wavelengths.

Вплив наночастинок золота на морфофункціональний стан гіпоталамуса та кори надниркових залоз щурів при ожирінні

The aim of our study was to compare the effects of a gold salt (sodium tetrachloroaurate) and gold nanoparticles colloidal solution on the morphofunctional state of the hypothalamus and adrenal glands of 6-month-old male rats with obesity, which was induced using a diet-induced model of metabolic disorders. The rats, modeled for obesity, were divided into three groups that received a NaCl solution, gold salt solution and colloidal solution of gold nanoparticles for 10 days. Rats of the control group received a standard diet. After the end of the experiment, the hypothalamic nuclei and adrenal glands, prepared according to the appropriate histological methods, were collected from the animals. In the paraventricular nucleus of the hypothalamus, a small-cell area of neurons was examined. In the adrenal glands, cells of three zones of the cortex were examined: glomerular, fascicular, and reticular. The results of the study showed that obesity led to the increase of functional activity in the nuclei of neurons in the small-cell region of the paraventricular nucleus of the rat hypothalamus. Functional activity increased maximally in the cells of the glomerular zone of the adrenal cortex, moderately increased in the fascicular zone, and no significant changes were recorded in the reticular zone. It was shown that the gold salt caused a decrease in the functional activity of neurons of the paraventricular nucleus of the hypothalamus in obese rats. In the glomerular zone of the adrenal cortex, the functional activity of cells significantly decreased; in the fascicular zone, the parameters of cell nuclei decreased to a greater extent; in the reticular zone, there were almost no changes. The introduction of gold nanoparticles colloidal solution caused the intensification of functional activity of the paraventricular hypothalamic nucleus cells in obese rats. In the glomerular zone of the adrenal cortex, cell parameters decreased; in the fascicular zone, cytoplasmic parameters decreased, but nuclear parameters did not change; in the reticular zone, measured values of nuclei increased and cytoplasmic parameters remained unchanged. In general, the administration of gold nanoparticles colloidal solution produced more pronounced effects than gold salt solution on cells of the paraventricular nucleus of the hypothalamus and the adrenal cortex in obese rats.

Spectroscopic properties of gold nanoparticles by laser irradiation

In this work a gold nanoparticles solutions has been irradiated by laser beam.The gold nanoparticles are prepared by using potassium bitarate as a reducing agent and Polyethylene glycol as a dispersion factor. The particles size of prepared solutions between 50-100 nanometers. Colloidal gold nanoparticles are irradiate with laser diode at period 5 and 10 minutes then the comparisons between two solutions with and without irradiation was done. The results are obtained by measuring the absorbance and using a spectrophotometer for the both solutions before and after irradiation. The result shows the differences between the spectrum of nanoparticles at ordinary state and spectrum of nanoparticles irradiated with laser beam. It's clear that the resolution is increase when the time of radiation is increasing. When spectrophotometers testing are done, we can note that there are broadening in the beginning of the spectrum of nanoparticles and increasing when time of exposure of laser beam is increasing.

Improvement of Proton Irradiation Effectiveness of Human Lung Carcinoma Cells A 549 in the Presence of Gold Nanoparticles

Purpose: To study was to study the effect of GNP nanoparticles on tumor cells of human lung carcinoma A 549 when irradiated with protons.
 Materials and methods:
 Cell culture: Human lung carcinoma cells A 549.
 Gold nanoparticles Au/PEG 6000/W 200/30 nm: A colloidal solution of gold nanoparticles purchased from the firm M 9 Nanomaterials & Technologies was used in the work.
 Proton irradiation: The irradiation of cells was carried out on a therapeutic proton beam in the Medical and Technical Complex of the Laboratory of Nuclear Problems Joint Institute for Nuclear Research.
 Determination of radiosensitivity of cells: By determining the clonogenic survival of cells.
 Determination of the genotoxic activity of nanoparticles under the action of proton irradiation: investigated using a micronucleus test with blocking cytokinesis. The genotoxicity of gold nanoparticles was estimated by the number of micronuclei per 1000 binuclear cells. Micronucleus were counted only in binuclear cells. At least 1000 binuclear cells were calculated for each dose, experiments were carried out in three repeats.
 Results: The frequency of micronucleus formation indicates an increase in the genotoxic effect of nanoparticles when irradiated with protons at a dose of 2 Gy. Proton irradiation caused an increase in the frequency of micronucleus formation depending on the concentration of nanoparticles. When irradiated with protons at a dose of 2 Gy in the presence and absence of nanoparticles, the difference in the frequency of micronucleus formation for the concentration of nanoparticles was 2.5 mg/ml – 1.1; for 5 mg/ml and 10 mg/ml – 1.2; for 15 mg/ml – 1.3 and for 30 mg/ml –1.5. Cell survival curves reflect a decrease in their survival rate when metal nanoparticles with high Z are added, which reflects the occurrence of radiosensitization effects. The gain for 10 % and 50 % survival rates is 1.4 and 2.5, respectively.
 Conclusions: Under the influence of proton irradiation, the genotoxic activity of gold nanoparticles in human lung carcinoma cells A 549 increases, depending on their concentration. The survival rate of human lung carcinoma A 549 cells irradiated with protons in the presence of gold nanoparticles decreases.

User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection.

To date, a range of nanozymes has been reported for their enzyme-mimicking catalytic activity such as solution-based sensors. However, in remote areas, the need for portable, cost-effective, and one-pot prepared sensors is obvious. In this study, we report the development of a highly stable and sensitive gold tablet-based sensor for cysteamine quantification in human serum samples. The sensor is produced in two steps: synthesis of a pullulan-stabilized gold nanoparticle solution (pAuNP-Solution) using a pullulan polymer as a reducing, stabilizing, and encapsulating agent and then, casting the pAuNP-Solution into a pullulan gold nanoparticle tablet (pAuNP-Tablet) by a pipetting method. The tablet was characterized by UV-vis, DLS, FTIR, TEM, and AFM analyses. The pAuNP-tablet exhibited a high peroxidase-mimetic activity via a TMB-H2O2 system. The presence of cysteamine in the system introduced two types of inhibition which were dependent on the cysteamine concentration. By determining Michaelis-Menten's kinetic parameters, we gained mechanistic insights into the catalytic inhibition process. Based on the catalytic inhibition capability of cysteamine, the limit of detection (LoD) was calculated to be 69.04 and 82.9 μM in buffer and human serum samples, respectively. Finally, real human serum samples were tested, demonstrating the applicability of the pAuNP-Tablet for real-world applications. The % R values in human serum samples were in the range of 91-105% with % RSD less than 2% for all replicas. The stability tests over 16 months revealed the ultra-stable properties of the pAuNP-Tablet. Overall, with a simple fabrication method and a novel employed technique, this study contributes to the advancement of tablet-based sensors and helps in cysteamine detection in clinical settings.

A Dihydropyridine Derivative as a Highly Selective Fluorometric Probe for Quantification of Au3+ Residue in Gold Nanoparticle Solution.

Novel dihydroquinoline derivatives (DHP and DHP-OH) were synthesized in one pot via a tandem trimerization-cyclization of methylpropiolate. DHP and DHP-OH possess strong blue fluorescence with high quantum efficiencies over 0.70 in aqueous media. DHP-OH displays a remarkable fluorescence quenching selectively to the presence of Au3+ through the oxidation of dihydropyridine to pyridinium ion as confirmed by NMR and HRMS. DHP-OH was used to demonstrate the quantitative analysis of Au3+ in water samples with the limit of detection of 33 ppb and excellent recovery (>95%). This fluorescent probe was also applied for the determination of Au3+ residue in the gold nanoparticle solution and a paper-based sensing strip for the on-site detection of Au3+.

Rapid Quantitative Detection of Voriconazole in Human Plasma Using Surface-Enhanced Raman Scattering.

There is an increasing demand for rapid detection techniques for monitoring the therapeutic concentration of voriconazole (VRC) in human biological fluids. Herein, a rapid and selective surface-enhanced Raman scatting method for point-of-care determination of VRC in human plasma was developed via a portable Raman spectrometer. This approach has enabled the quantification of the VRC spiked into human plasma at clinical relevant concentrations. A gold nanoparticle solution (Au sol) was used as the SERS substrate, and the agglomerating conditions on its sensitivity were optimized. The method involves the formation of hot spots, and the signal of VRC molecules adsorbed on the surface of the SERS hot spot was amplified by 105. The calibration curve was linear in the range of 0.02-10 ppm, with satisfactory repeatability. The limit of detection was as low as 12.3 ppb. The variation in VRC spectra over time on different substrates demonstrated good reproducibility. Notably, the salting-out extraction method developed in this study was rapid and suitable for the quantitation of drugs in biological samples. Compared with traditional methods, this approach allows for the point-of-care quantification of VRC directly in a complex matrix, which may open up new exciting opportunities for future use of the SERS technique in clinical applications.

Sử dụng hiện tượng cộng hưởng plasmon bề mặt của dung dịch nano vàng để xác định amikacin trong mẫu dược phẩm bằng phương pháp quang học

A simple and highly sensitive spectrophotometric method for detecting amikacin was developed based on the change in surface plasmon resonance (SPR) properties of gold nanoparticles (AuNPs). The change in the AuNPs solution colour from red to blue in the presence of trace amounts of amikacin makes the method visible to the naked eye. Furthermore, with the addition of amikacin, in the UV-Vis spectrum appears another absorption band at 650 nm, and the AuNPs solution also gave a linear increase of optical absorption ratio A650/A520 with a logarithm of amikacin concentration in the range of 3 × 10-7 - 3.5 × 10-6 M. Under the optimum conditions, including 2.5 × 10-4 M gold nanoparticles solution, pH = 6 and 2 mM NaCl with an incubation time of 4 minutes, the proposed method achieved the low limit of detection (LOD) of 7.2 × 10-8 M, the limit of quantitation (LOQ) of 2.4 × 10-7 M, high precision (RSD < 5%). Therefore, the developed method can be applied to determine amikacin in pharmaceutical samples and in plasma and urine samples.

Microcurrent and Gold Nanoparticles Combined with Hyaluronic Acid Accelerates Wound Healing.

This study aimed to investigate the effects of iontophoresis and hyaluronic acid (HA) combined with a gold nanoparticle (GNP) solution in an excisional wound model. Fifty Wistar rats (n = 10/group) were randomly assigned to the following groups: excisional wound (EW); EW + MC; EW + MC + HA; EW + MC + GNPs; and EW + MC + HA + GNPs. The animals were induced to a circular excision, and treatment started 24 h after injury with microcurrents (300 µA) containing gel with HA (0.9%) and/or GNPs (30 mg/L) in the electrodes (1 mL) for 7 days. The animals were euthanized 12 h after the last treatment application. The results demonstrate a reduction in the levels of pro-inflammatory cytokines (IFNϒ, IL-1β, TNFα, and IL-6) in the group in which the therapies were combined, and they show increased levels of anti-inflammatory cytokines (IL-4 and IL-10) and growth factors (FGF and TGF-β) in the EW + MC + HA and EW + MC + HA + GNPs groups. As for the levels of dichlorofluorescein (DCF) and nitrite, as well as oxidative damage (carbonyl and sulfhydryl), they decreased in the combined therapy group when compared to the control group. Regarding antioxidant defense, there was an increase in glutathione (GSH) and a decrease in superoxide dismutase (SOD) in the combined therapy group. A histological analysis showed reduced inflammatory infiltrate in the MC-treated groups and in the combination therapy group. There was an increase in the wound contraction rate in all treated groups when compared to the control group, proving that the proposed therapies are effective in the epithelial healing process. The results of this study demonstrate that the therapies in combination favor the tissue repair process more significantly than the therapies in isolation.

Study of the Applicability of Colloidal Solutions of Gold and Silver Nanoparticles for Surface-enhanced Raman Spectroscopy of Blood Plasma

This work focuses on the preparation of colloidal solutions of Au and Ag nanoparticles and verification of their applicability for surface-enhanced Raman scattering spectroscopy of blood plasma. The composition of blood plasma may change due to pathobiochemical processes associated with disease development. Analysis of blood plasma might reveal these changes and thus help in the diagnostics process of various diseases. Raman spectroscopy appears to be an ideal method for rapid and non-destructive analysis of blood plasma. Moreover, surface-enhanced Raman scattering spectroscopy might allow the detection of very low levels of potential disease-specific markers. In this work, the prepared colloidal solutions of Au and Ag nanoparticles were first characterized by UV‑VIS absorption spectroscopy and their enhancing capabilities were verified using riboflavin as a model analyte. Subsequently, optimization of the measurement conditions for surface-enhanced Raman spectroscopy of blood plasma was performed. The results show that at the excitation wavelength of 785 nm, significantly higher enhancement is achieved when using Ag nanoparticles. In the case of Au nanoparticles, the enhanced blood plasma spectrum was successfully detected only once, and the addition of an aggregating agent (NaCl) was required.

All-Optical Tunability of Metalenses Permeated with Liquid Crystals.

Metasurfaces have been extensively engineered to produce a wide range of optical phenomena, allowing exceptional control over the propagation of light. However, they are generally designed as single-purpose devices without a modifiable postfabrication optical response, which can be a limitation to real-world applications. In this work, we report a nanostructured planar-fused silica metalens permeated with a nematic liquid crystal (NLC) and gold nanoparticle solution. The physical properties of embedded NLCs can be manipulated with the application of external stimuli, enabling reconfigurable optical metasurfaces. We report the all-optical, dynamic control of the metalens optical response resulting from thermoplasmonic-induced changes of the NLC solution associated with the nematic-isotropic phase transition. A continuous and reversible tuning of the metalens focal length is experimentally demonstrated, with a variation of 80 μm (0.16% of the 5 cm nominal focal length) along the optical axis. This is achieved without direct mechanical or electrical manipulation of the device. The reconfigurable properties are compared with corroborating numerical simulations of the focal length shift and exhibit close correspondence.

Observation and biomedical application of plasmon-enhanced fluorescence induced multiple stimulated Stokes Raman scattering in FITC-conjugated gold nanoparticles solution

This work describes the observation of multi-wavelengths due to stimulated Raman scattering from deep red to near-infrared (NIR) induced by plasmon-enhanced fluorescence (PEF) of fluorescein isothiocyanate (FITC) conjugated gold nanoparticles (F-AuNP) excited by 488 nm diode laser. Coupling between the near-field of localized surface plasmons of AuNP with molecules amplifies the fluorescence signal i.e. brightness hence the quantum yield by several orders of magnitude. Resonance Rayleigh scattering and PEF conditions are satisfied due to the overlapping of 488 nm of the laser beam with the maximum absorbance of FITC, and the SPR of AuNP with FITC emission spectrum at ≈525 nm. PEF acts as Stoke sand secondary pump beam for further optical excitation. Surface-enhanced Raman scattering (SERS) showed FITC molecular bonds at 600, 1680, and a weak deviation at 1280 cm−1, respectively. Longer wavelengths with higher output power were observed between 180° and 270°, and shorter wavelengths with lower power between 90° and 0° respectively. F-AuNPs were incubated and up taken by the oyster mushroom (OM) grown in the lab for bioimaging purposes and studied by phase-contrast microscope (PCM), and fluorescence microscope (FM). The FM results revealed visible colours, which can be utilized for in vitro and in vivo biosensing applications.